AlanFung:LabNotes/Cell/2009-10-11: Difference between revisions

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>Alan6017518
(New page: ==MEF== *Make 3x10cm MEF plates for HUES MCM *Make 3x10cm MEF plates for Wicell MCM ==HUES9== *Feed 1x10cm (matrigel) + 1 well (mef) w/ 20% HUES MCM ==H9== *Feed 1x10cm (matrigel) + 3 x ...)
 
>Alan6017518
 
(One intermediate revision by the same user not shown)
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==HUES9==
==HUES9==
*Feed 1x10cm (matrigel) + 1 well (mef) w/ 20% HUES MCM
*Feed 1x10cm (matrigel) + 1 well (mef) w/ 20% HUES MCM
*[[Media:SNAP-201933-0002.JPG]]


==H9==
==H9==
*Feed 1x10cm (matrigel) + 3 x 6 wells (mef) w/ 100% wicell mcm
*Feed 1x10cm (matrigel) + 3 x 6 wells (mef) w/ 100% wicell mcm
*[[Media:SNAP-202118-0003.JPG]]


==HUES53==
==HUES53==

Latest revision as of 18:18, 12 October 2009

MEF[edit]

  • Make 3x10cm MEF plates for HUES MCM
  • Make 3x10cm MEF plates for Wicell MCM

HUES9[edit]

H9[edit]

HUES53[edit]

  • Cells are ~80% confluent, split 1x10cm (matrigel) (1:2) to 2x10cm (matrigel) w/100% HUES MCM
  • Feed 3 wells (mef) w/ 20% HUES MCM
  • Cells are probably ready to collect on Monday

Matrigel[edit]