Baylor-exon-Sept09: Difference between revisions

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>Jbrubake
(New page: '''Oligo Separation''' 1x 48x Template 0.4ul 19.2ul 2x Kapa Master Mix 50ul 50ul...)
 
>Jbrubake
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'''Oligo Separation'''
===Baylor (BCM) Creation===


'''PCR'''
                                           1x              48x
                                           1x              48x
Template                        0.4ul        19.2ul
Template                        0.4ul        19.2ul
Line 7: Line 8:
eMIP-CA1F (100uM)    0.4ul          0.4ul
eMIP-CA1F (100uM)    0.4ul          0.4ul
eMIP-CA1R (100uM)    0.4ul          0.4ul
eMIP-CA1R (100uM)    0.4ul          0.4ul
'''PCR:''' 95C, 30sec -> (95C, 3sec -> 54C, 30sec -> 60C, 20sec -> plate read) x 25 -> 60C, 2min -> 15C hold
PCR: 95C, 30sec -> (95C, 3sec -> 54C, 30sec -> 60C, 20sec -> plate read) x 25 -> 60C, 2min -> 15C hold


*Note: Amplification started after ~13 cycles
*Note: Amplification started after ~13 cycles

Revision as of 02:14, 4 November 2009

Baylor (BCM) Creation

PCR

                                          1x               48x

Template 0.4ul 19.2ul 2x Kapa Master Mix 50ul 50ul H2O 50ul 50ul eMIP-CA1F (100uM) 0.4ul 0.4ul eMIP-CA1R (100uM) 0.4ul 0.4ul PCR: 95C, 30sec -> (95C, 3sec -> 54C, 30sec -> 60C, 20sec -> plate read) x 25 -> 60C, 2min -> 15C hold

  • Note: Amplification started after ~13 cycles