Baylor-exon-Sept09: Difference between revisions
Jump to navigation
Jump to search
>Jbrubake (New page: '''Oligo Separation''' 1x 48x Template 0.4ul 19.2ul 2x Kapa Master Mix 50ul 50ul...) |
>Jbrubake No edit summary |
||
Line 1: | Line 1: | ||
===Baylor (BCM) Creation=== | |||
'''PCR''' | |||
1x 48x | 1x 48x | ||
Template 0.4ul 19.2ul | Template 0.4ul 19.2ul | ||
Line 7: | Line 8: | ||
eMIP-CA1F (100uM) 0.4ul 0.4ul | eMIP-CA1F (100uM) 0.4ul 0.4ul | ||
eMIP-CA1R (100uM) 0.4ul 0.4ul | eMIP-CA1R (100uM) 0.4ul 0.4ul | ||
PCR: 95C, 30sec -> (95C, 3sec -> 54C, 30sec -> 60C, 20sec -> plate read) x 25 -> 60C, 2min -> 15C hold | |||
*Note: Amplification started after ~13 cycles | *Note: Amplification started after ~13 cycles |
Revision as of 02:14, 4 November 2009
Baylor (BCM) Creation
PCR
1x 48x
Template 0.4ul 19.2ul 2x Kapa Master Mix 50ul 50ul H2O 50ul 50ul eMIP-CA1F (100uM) 0.4ul 0.4ul eMIP-CA1R (100uM) 0.4ul 0.4ul PCR: 95C, 30sec -> (95C, 3sec -> 54C, 30sec -> 60C, 20sec -> plate read) x 25 -> 60C, 2min -> 15C hold
- Note: Amplification started after ~13 cycles