Baylor-exon-Sept09: Difference between revisions
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'''PCR''' | '''PCR''' | ||
1x 48x | |||
Template 0.4ul 19.2ul | Template 0.4ul 19.2ul | ||
2x Kapa Master Mix 50ul 50ul | 2x Kapa Master Mix 50ul 50ul | ||
H2O 50ul 50ul | H2O 50ul 50ul | ||
eMIP-CA1F (100uM) 0.4ul 0.4ul | eMIP-CA1F (100uM) 0.4ul 0.4ul | ||
eMIP-CA1R (100uM) 0.4ul 0.4ul | eMIP-CA1R (100uM) 0.4ul 0.4ul | ||
PCR: 95C, 30sec -> (95C, 3sec -> 54C, 30sec -> 60C, 20sec -> plate read) x 25 -> 60C, 2min -> 15C hold | PCR: 95C, 30sec -> (95C, 3sec -> 54C, 30sec -> 60C, 20sec -> plate read) x 25 -> 60C, 2min -> 15C hold | ||
*Note: Amplification started after ~13 cycles | *Note: Amplification started after ~13 cycles |
Revision as of 02:15, 4 November 2009
Baylor (BCM) Creation
PCR
1x 48x Template 0.4ul 19.2ul 2x Kapa Master Mix 50ul 50ul H2O 50ul 50ul eMIP-CA1F (100uM) 0.4ul 0.4ul eMIP-CA1R (100uM) 0.4ul 0.4ul PCR: 95C, 30sec -> (95C, 3sec -> 54C, 30sec -> 60C, 20sec -> plate read) x 25 -> 60C, 2min -> 15C hold
- Note: Amplification started after ~13 cycles