Baylor-exon-Sept09: Difference between revisions

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'''PCR'''
'''PCR'''
                                            1x              48x
                          1x              48x
  Template                         0.4ul         19.2ul  
  Template               0.4ul           19.2ul  
  2x Kapa Master Mix       50ul            50ul
  2x Kapa Master Mix     50ul            50ul
  H2O                                 50ul            50ul
  H2O                     50ul            50ul
  eMIP-CA1F (100uM)     0.4ul           0.4ul
  eMIP-CA1F (100uM)     0.4ul           0.4ul
  eMIP-CA1R (100uM)     0.4ul         0.4ul
  eMIP-CA1R (100uM)     0.4ul           0.4ul
  PCR: 95C, 30sec -> (95C, 3sec -> 54C, 30sec -> 60C, 20sec -> plate read) x 25 -> 60C, 2min -> 15C hold
  95C, 30sec -> (95C, 3sec -> 54C, 30sec -> 60C, 20sec -> plate read) x 25 -> 60C, 2min -> 15C hold


*Note: Amplification started after ~13 cycles
*Note: Amplification started after ~13 cycles

Revision as of 02:18, 4 November 2009

Baylor (BCM) Creation

PCR

                         1x               48x
Template               0.4ul           19.2ul 
2x Kapa Master Mix      50ul             50ul
H2O                     50ul             50ul
eMIP-CA1F (100uM)      0.4ul            0.4ul
eMIP-CA1R (100uM)      0.4ul            0.4ul
95C, 30sec -> (95C, 3sec -> 54C, 30sec -> 60C, 20sec -> plate read) x 25 -> 60C, 2min -> 15C hold
  • Note: Amplification started after ~13 cycles