CES36k18bp: Difference between revisions

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>Jbrubake
(New page: ===CES36k18bp (CES) Creation===)
 
>Jbrubake
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===CES36k18bp (CES) Creation===
===CES36k18bp (CES) Creation===
'''PCR'''
                          1x              48x
Template              0.4ul          19.2ul
2x Kapa Master Mix      50ul            50ul
H2O                    50ul            50ul
AP1V61U (100uM)      0.4ul            0.4ul
AP2V6 (100uM)      0.4ul            0.4ul
95C, 30sec -> (95C, 3sec ->  60C, 50sec -> plate read) x 25 -> 60C, 2min -> 15C hold

Revision as of 18:25, 4 November 2009

CES36k18bp (CES) Creation

PCR

                         1x               48x
Template               0.4ul           19.2ul 
2x Kapa Master Mix      50ul             50ul
H2O                     50ul             50ul
AP1V61U (100uM)      0.4ul            0.4ul
AP2V6 (100uM)      0.4ul            0.4ul
95C, 30sec -> (95C, 3sec ->  60C, 50sec -> plate read) x 25 -> 60C, 2min -> 15C hold