CES36k18bp: Difference between revisions

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>Jbrubake
>Jbrubake
Line 9: Line 9:
  AP2V6 (100uM)          0.4ul            0.4ul
  AP2V6 (100uM)          0.4ul            0.4ul
  95C, 30sec -> (95C, 3sec ->  60C, 50sec -> plate read) x 25 -> 60C, 2min -> 15C hold
  95C, 30sec -> (95C, 3sec ->  60C, 50sec -> plate read) x 25 -> 60C, 2min -> 15C hold
*Note: Amplification stated at ~12 cycles
'''Ethanol Precipitation'''

Revision as of 18:27, 4 November 2009

CES36k18bp (CES) Creation

PCR

                         1x               48x
Template               0.4ul           19.2ul 
2x Kapa Master Mix      50ul             50ul
H2O                     50ul             50ul
AP1V61U (100uM)        0.4ul            0.4ul
AP2V6 (100uM)          0.4ul            0.4ul
95C, 30sec -> (95C, 3sec ->  60C, 50sec -> plate read) x 25 -> 60C, 2min -> 15C hold
  • Note: Amplification stated at ~12 cycles

Ethanol Precipitation