CES36k18bp: Difference between revisions
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>Jbrubake |
>Jbrubake |
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AP2V6 (100uM) 0.4ul 0.4ul | AP2V6 (100uM) 0.4ul 0.4ul | ||
95C, 30sec -> (95C, 3sec -> 60C, 50sec -> plate read) x 25 -> 60C, 2min -> 15C hold | 95C, 30sec -> (95C, 3sec -> 60C, 50sec -> plate read) x 25 -> 60C, 2min -> 15C hold | ||
*Note: Amplification stated at ~12 cycles | |||
'''Ethanol Precipitation''' |
Revision as of 18:27, 4 November 2009
CES36k18bp (CES) Creation
PCR
1x 48x Template 0.4ul 19.2ul 2x Kapa Master Mix 50ul 50ul H2O 50ul 50ul AP1V61U (100uM) 0.4ul 0.4ul AP2V6 (100uM) 0.4ul 0.4ul 95C, 30sec -> (95C, 3sec -> 60C, 50sec -> plate read) x 25 -> 60C, 2min -> 15C hold
- Note: Amplification stated at ~12 cycles
Ethanol Precipitation