AlanFung:LabNotes/Sequencing/2009-11-11: Difference between revisions
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>Alan6017518 (New page: ==Rescue CVF-N==) |
>Alan6017518 |
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==Rescue CVF-N== | ==Rescue CVF-N== | ||
*20ul of CVF-N library, with buffer added instead of iProof master mix | |||
==Ethanol Precipitation== | |||
*Mix 50ul 100% ethanol, 2ul 3M NaoAC, and 1ul glycoblue | |||
*Store at -80C for 20min | |||
*Centrifuge 10,000 for 20min at 4C | |||
*Discard supernatant | |||
*Add in 750ul 75%ethanol | |||
*Spin 10,000 rpm for 5 min at 4C | |||
*Discard supernatant | |||
*let dry in hood for 10 min | |||
*Add in 21ul ddh2o | |||
Repeat PCR of sequencing library with the following sets |
Revision as of 20:36, 11 November 2009
Rescue CVF-N
- 20ul of CVF-N library, with buffer added instead of iProof master mix
Ethanol Precipitation
- Mix 50ul 100% ethanol, 2ul 3M NaoAC, and 1ul glycoblue
- Store at -80C for 20min
- Centrifuge 10,000 for 20min at 4C
- Discard supernatant
- Add in 750ul 75%ethanol
- Spin 10,000 rpm for 5 min at 4C
- Discard supernatant
- let dry in hood for 10 min
- Add in 21ul ddh2o
Repeat PCR of sequencing library with the following sets