AlanFung:LabNotes/Sequencing/2009-11-11: Difference between revisions

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>Alan6017518
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==Work on Set 8 DF, 8 FS, 9 DF, 9 FS, 8 PGP1-iPS==
==Work on Set 8 DF, 8 FS, 9 DF, 9 FS, 8 PGP1-iPS==
*Start from Size Selection
*Start from Size Selection
[[Image:ZhangLab_2 2009-11-11 14hr 28min.jpg]]
[[Image:ZhangLab_2 2009-11-11 14hr 30min.jpg]]

Revision as of 18:43, 12 November 2009

Rescue CVF-N

  • 20ul of CVF-N library, with buffer added instead of iProof master mix

Ethanol Precipitation

  • Mix 50ul 100% ethanol, 2ul 3M NaoAC, and 1ul glycoblue
  • Store at -80C for 20min
  • Centrifuge 10,000 for 20min at 4C
  • Discard supernatant
  • Add in 750ul 75%ethanol
  • Spin 10,000 rpm for 5 min at 4C
  • Discard supernatant
  • let dry in hood for 10 min
  • Add in 21ul ddh2o
Repeat PCR of sequencing library with the following sets

Work on Set 8 DF, 8 FS, 9 DF, 9 FS, 8 PGP1-iPS

  • Start from Size Selection

File:ZhangLab 2 2009-11-11 14hr 28min.jpg File:ZhangLab 2 2009-11-11 14hr 30min.jpg