AlanFung:LabNotes/Sequencing/2009-11-11: Difference between revisions
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==Work on Set 8 DF, 8 FS, 9 DF, 9 FS, 8 PGP1-iPS== | ==Work on Set 8 DF, 8 FS, 9 DF, 9 FS, 8 PGP1-iPS== | ||
*Start from Size Selection | *Start from Size Selection | ||
[[Image:ZhangLab_2 2009-11-11 14hr 28min.jpg]] | |||
[[Image:ZhangLab_2 2009-11-11 14hr 30min.jpg]] |
Revision as of 18:43, 12 November 2009
Rescue CVF-N
- 20ul of CVF-N library, with buffer added instead of iProof master mix
Ethanol Precipitation
- Mix 50ul 100% ethanol, 2ul 3M NaoAC, and 1ul glycoblue
- Store at -80C for 20min
- Centrifuge 10,000 for 20min at 4C
- Discard supernatant
- Add in 750ul 75%ethanol
- Spin 10,000 rpm for 5 min at 4C
- Discard supernatant
- let dry in hood for 10 min
- Add in 21ul ddh2o
Repeat PCR of sequencing library with the following sets
Work on Set 8 DF, 8 FS, 9 DF, 9 FS, 8 PGP1-iPS
- Start from Size Selection
File:ZhangLab 2 2009-11-11 14hr 28min.jpg File:ZhangLab 2 2009-11-11 14hr 30min.jpg