AlanFung:LabNotes/Sequencing/2009-11-22: Difference between revisions
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>Alan6017518 (→CVF-N) |
>Alan6017518 (→CVF-N) |
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*Reamplify library and ship it out for shearing | *Reamplify library and ship it out for shearing | ||
{| {{table}} | {| {{table}} | ||
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Revision as of 05:35, 23 November 2009
CVF-N
- Reamplify library and ship it out for shearing
' | ' | x4 | X1.1 | ||||||
Library | 1 | - | - | - | 2X Phusion | 50 | 750 | 825 | |
AmpF6.3NH2 (100uM) | 2 | 3 | 3.3 | ||||||
AmpR6.3NH2 (100uM) | 2 | 3 | 3.3 | ||||||
50X SYBR Green I | 2 | 3 | 3.3 | ||||||
dH2O | 32 | 645 | 709.5 | ||||||
- 98C 1min->6X(98C 10 sec, 58C 20 sec, 72C 20sec)->72C 5 min