AlanFung:LabNotes/Sequencing/2009-11-22: Difference between revisions

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>Alan6017518
>Alan6017518
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| align="center" style="background:#f0f0f0;"|'''X1.1'''
| align="center" style="background:#f0f0f0;"|'''X1.1'''
|-
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| Library||1||-||-||-
| Library||1||-||-||
|-
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| 2X Phusion||50||750||825
| 2X Phusion||50||750||825

Revision as of 05:36, 23 November 2009

CVF-N

  • Reamplify library and ship it out for shearing
' ' x4 X1.1
Library 1 - -
2X Phusion 50 750 825
AmpF6.3NH2 (100uM) 2 3 3.3
AmpR6.3NH2 (100uM) 2 3 3.3
50X SYBR Green I 2 3 3.3
dH2O 32 645 709.5
  • 98C 1min->6X(98C 10 sec, 58C 20 sec, 72C 20sec)->72C 5 min