AlanFung:LabNotes/Sequencing/2009-11-22: Difference between revisions

From ZhangLabWiki
Jump to navigation Jump to search
>Alan6017518
>Alan6017518
Line 11: Line 11:
| 2X Phusion||50||750||825
| 2X Phusion||50||750||825
|-
|-
| AmpF6.3NH2 (100uM)||2||3||3.3
| AmpF6.3NH2 (100uM)||0.2||3||3.3
|-
|-
| AmpR6.3NH2 (100uM)||2||3||3.3
| AmpR6.3NH2 (100uM)||0.2||3||3.3
|-
|-
| 50X SYBR Green I||2||3||3.3
| 50X SYBR Green I||0.2||3||3.3
|-
|-
| dH2O||32||645||709.5
| dH2O||43||645||709.5
|-
|-
|  
|  

Revision as of 05:37, 23 November 2009

CVF-N

  • Reamplify library and ship it out for shearing
' ' x15 X1.1
Library 1 - -
2X Phusion 50 750 825
AmpF6.3NH2 (100uM) 0.2 3 3.3
AmpR6.3NH2 (100uM) 0.2 3 3.3
50X SYBR Green I 0.2 3 3.3
dH2O 43 645 709.5
  • 98C 1min->6X(98C 10 sec, 58C 20 sec, 72C 20sec)->72C 5 min