AlanFung:LabNotes/Sequencing/2009-11-22: Difference between revisions
Jump to navigation
Jump to search
>Alan6017518 (→CVF-N) |
>Alan6017518 (→CVF-N) |
||
Line 11: | Line 11: | ||
| 2X Phusion||50||750||825 | | 2X Phusion||50||750||825 | ||
|- | |- | ||
| AmpF6.3NH2 (100uM)||2||3||3.3 | | AmpF6.3NH2 (100uM)||0.2||3||3.3 | ||
|- | |- | ||
| AmpR6.3NH2 (100uM)||2||3||3.3 | | AmpR6.3NH2 (100uM)||0.2||3||3.3 | ||
|- | |- | ||
| 50X SYBR Green I||2||3||3.3 | | 50X SYBR Green I||0.2||3||3.3 | ||
|- | |- | ||
| dH2O|| | | dH2O||43||645||709.5 | ||
|- | |- | ||
| | | |
Revision as of 05:37, 23 November 2009
CVF-N
- Reamplify library and ship it out for shearing
' | ' | x15 | X1.1 | |
Library | 1 | - | - | |
2X Phusion | 50 | 750 | 825 | |
AmpF6.3NH2 (100uM) | 0.2 | 3 | 3.3 | |
AmpR6.3NH2 (100uM) | 0.2 | 3 | 3.3 | |
50X SYBR Green I | 0.2 | 3 | 3.3 | |
dH2O | 43 | 645 | 709.5 | |
- 98C 1min->6X(98C 10 sec, 58C 20 sec, 72C 20sec)->72C 5 min