AlanFung:LabNotes/Sequencing/2009-11-22: Difference between revisions
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>Alan6017518 (→CVF-N) |
>Alan6017518 (→CVF-N) |
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| align="center" style="background:#f0f0f0;"|'''x15''' | | align="center" style="background:#f0f0f0;"|'''x15''' | ||
| align="center" style="background:#f0f0f0;"|'''X1.1''' | | align="center" style="background:#f0f0f0;"|'''X1.1''' | ||
| align="center" style="background:#f0f0f0;"|'''/2''' | |||
|- | |- | ||
| Library||1||-||-|| | | Library||1||-||-||-|| | ||
|- | |- | ||
| 2X Phusion||50||750||825 | | 2X Phusion||50||750||825||411.5 | ||
|- | |- | ||
| AmpF6.3NH2 (100uM)||0.2||3||3.3 | | AmpF6.3NH2 (100uM)||0.2||3||3.3||1.65 | ||
|- | |- | ||
| AmpR6.3NH2 (100uM)||0.2||3||3.3 | | AmpR6.3NH2 (100uM)||0.2||3||3.3||1.65 | ||
|- | |- | ||
| 50X SYBR Green I||0.2||3||3.3 | | 50X SYBR Green I||0.2||3||3.3||1.65 | ||
|- | |- | ||
| dH2O||43||645||709.5 | | dH2O||43||645||709.5||354.75 | ||
|- | |- | ||
| | | |
Revision as of 05:38, 23 November 2009
CVF-N
- Reamplify library and ship it out for shearing
' | ' | x15 | X1.1 | /2 | |
Library | 1 | - | - | - | |
2X Phusion | 50 | 750 | 825 | 411.5 | |
AmpF6.3NH2 (100uM) | 0.2 | 3 | 3.3 | 1.65 | |
AmpR6.3NH2 (100uM) | 0.2 | 3 | 3.3 | 1.65 | |
50X SYBR Green I | 0.2 | 3 | 3.3 | 1.65 | |
dH2O | 43 | 645 | 709.5 | 354.75 | |
- 98C 1min->6X(98C 10 sec, 58C 20 sec, 72C 20sec)->72C 5 min