AlanFung:LabNotes/Sequencing/2009-11-22: Difference between revisions
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>Alan6017518 (→CVF-N) |
>Alan6017518 (→CVF-N) |
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==CVF-N== | ==CVF-N== | ||
*Reamplify library and ship it out for shearing | *Reamplify library and ship it out for shearing | ||
A-22.5ng/ul | |||
B-12.8ng/ul | |||
C-6ng/ul | |||
D-5.4ng/ul | |||
A 2ul | |||
B 1.76ul | |||
C 5.48ul | |||
D 32.85ul | |||
Total 15.32ul | |||
*Amplify in 15 wells | |||
{| {{table}} | {| {{table}} | ||
| align="center" style="background:#f0f0f0;"|'''''' | | align="center" style="background:#f0f0f0;"|'''''' |
Revision as of 06:01, 23 November 2009
CVF-N
- Reamplify library and ship it out for shearing
A-22.5ng/ul B-12.8ng/ul C-6ng/ul D-5.4ng/ul
A 2ul B 1.76ul C 5.48ul D 32.85ul Total 15.32ul
- Amplify in 15 wells
' | ' | x15 | X1.1 | /2 | |
Library | 1 | - | - | 7.66 | |
2X Phusion | 50 | 750 | 825 | 412.5 | |
AmpF6.3NH2 (100uM) | 0.2 | 3 | 3.3 | 1.65 | |
AmpR6.3NH2 (100uM) | 0.2 | 3 | 3.3 | 1.65 | |
50X SYBR Green I | 0.2 | 3 | 3.3 | 1.65 | |
dH2O | 43 | 645 | 709.5 | 354.75 | |
- 98C 1min->6X(98C 10 sec, 58C 20 sec, 72C 20sec)->72C 5 min