AlanFung:Protocol/Construction of Solexa sequencing Library(USER): Difference between revisions
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>Alan6017518 (New page: ==Sequencing Library Construction with Single Adaptor Protocol== ===PCR with dUTP and 6.3NH2 Primer=== {| {{table}} | ||1 well |- | H2O||47.4 |- | Amp F6.3NH2(100uM)||0.2 |- | Amp R6.3NH2 ...) |
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==Sequencing Library Construction with Single Adaptor Protocol== | ==Sequencing Library Construction with Single Adaptor Protocol== | ||
==PCR with dUTP and 6.3NH2 Primer== | |||
{| {{table}} | {| {{table}} | ||
| ||1 well | | ||1 well |
Revision as of 16:36, 24 November 2009
Sequencing Library Construction with Single Adaptor Protocol
PCR with dUTP and 6.3NH2 Primer
1 well | |
H2O | 47.4 |
Amp F6.3NH2(100uM) | 0.2 |
Amp R6.3NH2 (100uM) | 0.2 |
dUTP (1mM) | 0.8 |
50X SYBG I | 0.4 |
2X Master Mix | 50 |
Template | 1 |
Total | 100 |