AlanFung:Protocol/Construction of Solexa sequencing Library(USER): Difference between revisions
Jump to navigation
Jump to search
>Alan6017518 |
>Alan6017518 |
||
Line 22: | Line 22: | ||
| | | | ||
|} | |} | ||
==USER Digestion== |
Revision as of 16:36, 24 November 2009
Sequencing Library Construction with Single Adaptor Protocol
PCR with dUTP and 6.3NH2 Primer
1 well | |
H2O | 47.4 |
Amp F6.3NH2(100uM) | 0.2 |
Amp R6.3NH2 (100uM) | 0.2 |
dUTP (1mM) | 0.8 |
50X SYBG I | 0.4 |
2X Master Mix | 50 |
Template | 1 |
Total | 100 |