AlanFung:Protocol/Construction of Solexa sequencing Library(USER): Difference between revisions

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*Qiaquick purification, elute with 32ul
==USER Digestion==
==USER Digestion==
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Revision as of 16:37, 24 November 2009

Sequencing Library Construction with Single Adaptor Protocol

PCR with dUTP and 6.3NH2 Primer

1 well
H2O 47.4
Amp F6.3NH2(100uM) 0.2
Amp R6.3NH2 (100uM) 0.2
dUTP (1mM) 0.8
50X SYBG I 0.4
2X Master Mix 50
Template 1
Total 100
  • Qiaquick purification, elute with 32ul

USER Digestion

DNA 30
USER 3
Total 33