Dinh:LAB/Bisulfite Sequencing: Difference between revisions
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*[[Dinh:Protocols/Probes_Prep_Nov17| Nov 17 2009 Probes Preparation]], CpG30K, 3 plates of 96rxns, Taq2xMM from NEB, Nanodrop: 100ng/ul (72ug). | *[[Dinh:Protocols/Probes_Prep_Nov17| Nov 17 2009 Probes Preparation]], CpG30K, 3 plates of 96rxns, Taq2xMM from NEB, Nanodrop: 100ng/ul (72ug). | ||
*[[Dinh:Protocols/Probes_Prep_Nov24| Nov 24 2009 Probes Preparation]], CpG30K, KAPA qPCR kit from KAPA Biosystems. | *[[Dinh:Protocols/Probes_Prep_Nov24| Nov 24 2009 Probes Preparation]], CpG30K, KAPA qPCR kit from KAPA Biosystems, Nanodrop: 21.4 ug, End point PAGE: 35ng/ul x 20 ul | ||
=Capturing= | =Capturing= | ||
* [[Dinh:Protocols/Capturing_Nov13| Nov 13 2009 Capturing - Practice]], CpG30K, Positive control: Jurkat, Negative control: RNAse free-ddH20 | * [[Dinh:Protocols/Capturing_Nov13| Nov 13 2009 Capturing - Practice]], CpG30K, Positive control: Jurkat, Negative control: RNAse free-ddH20 |
Revision as of 22:49, 4 December 2009
<calendar> name=Dinh format=%name/NOTES/%year-%month-%day date=2009/07/01 view=oneyear </calendar>
Probes Preparation
- Sep 28 2009 Practice Probes Preparation, CpG30K, 24rxns, Nanodrop: 4.64 ug, End Point Gel Quantification: 1.91 ug
- Oct 1 2009 Probes Preparation, CpG30K, 24rxns, Nanodrop:, End Point Gel Quantification: Problem with 2D gel
- Oct 6 2009 Probes Preparation, CpG30K, 96rxns x 3, Nanodrop: 22.2 ug (last plate: Master Cycler), 55.5 ug (RT-BioRad), 51.5 ug (BioRad). End Point Gel Quantification: 13ng/ul x 46ul
- Oct 27 2009 Probes Preparation, CpG30K, 96rxns, Nanodrop: 67.12 ng/ul (32.2 ug). End Point Gel Quantification: 12ng/ul x 20ul
- Nov 2 2009 Probes Preparation, CpG30K, 96rxns, Nanodrop: 115ng/ul (57.5 ug). End Point Gel Quantification: 7ng/ul x 11ul
- Nov 6 2009 Probes Preparation, CpG30K, 96rxns, Nanodrop: 120ng/ul (60.0 ug). End Point Gel Quantification: 12ng/ul x 22ul
- Nov 17 2009 Probes Preparation, CpG30K, 3 plates of 96rxns, Taq2xMM from NEB, Nanodrop: 100ng/ul (72ug).
- Nov 24 2009 Probes Preparation, CpG30K, KAPA qPCR kit from KAPA Biosystems, Nanodrop: 21.4 ug, End point PAGE: 35ng/ul x 20 ul
Capturing
- Nov 13 2009 Capturing - Practice, CpG30K, Positive control: Jurkat, Negative control: RNAse free-ddH20