Dinh:LAB/Bisulfite Sequencing: Difference between revisions

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*[[Dinh:Protocols/Probes_Prep_Nov17| Nov 17 2009 Probes Preparation]], CpG30K, 3 plates of 96rxns, Taq2xMM from NEB, Nanodrop: 100ng/ul (72ug).
*[[Dinh:Protocols/Probes_Prep_Nov17| Nov 17 2009 Probes Preparation]], CpG30K, 3 plates of 96rxns, Taq2xMM from NEB, Nanodrop: 100ng/ul (72ug).


*[[Dinh:Protocols/Probes_Prep_Nov24| Nov 24 2009 Probes Preparation]], CpG30K, KAPA qPCR kit from KAPA Biosystems.
*[[Dinh:Protocols/Probes_Prep_Nov24| Nov 24 2009 Probes Preparation]], CpG30K, KAPA qPCR kit from KAPA Biosystems, Nanodrop: 21.4 ug, End point PAGE: 35ng/ul x 20 ul


=Capturing=
=Capturing=
* [[Dinh:Protocols/Capturing_Nov13| Nov 13 2009 Capturing - Practice]], CpG30K, Positive control: Jurkat, Negative control: RNAse free-ddH20
* [[Dinh:Protocols/Capturing_Nov13| Nov 13 2009 Capturing - Practice]], CpG30K, Positive control: Jurkat, Negative control: RNAse free-ddH20

Revision as of 22:49, 4 December 2009

<calendar> name=Dinh format=%name/NOTES/%year-%month-%day date=2009/07/01 view=oneyear </calendar>

Probes Preparation

Capturing