Dinh:LAB/Bisulfite Sequencing: Difference between revisions

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*[[Dinh:Protocols/Probes_Prep_Nov17| Nov 17 2009 Probes Preparation]], CpG30K, 3 plates of 96rxns, Taq2xMM from NEB, Nanodrop: 100ng/ul (72ug).
*[[Dinh:Protocols/Probes_Prep_Nov17| Nov 17 2009 Probes Preparation]], CpG30K, 3 plates of 96rxns, Taq2xMM from NEB, Nanodrop: 100ng/ul (72ug).


*[[Dinh:Protocols/Probes_Prep_Nov24| Nov 24 2009 Probes Preparation]], CpG30K, KAPA qPCR kit from KAPA Biosystems, Nanodrop: 21.4 ug, End point PAGE: 23ng/ul x 20 ul
*[[Dinh:Protocols/Probes_Prep_Nov24| Nov 24 2009 Probes Preparation]], CpG30K, KAPA qPCR kit from KAPA Biosystems, Nanodrop: 21.4 ug, End point [http://genome-tech.ucsd.edu/LabNotes/index.php/Dinh/NOTES/2009-12-4 QUBIT]: 23ng/ul x 20 ul


*[http://genome-tech.ucsd.edu/LabNotes/index.php/Dinh/NOTES/2009-12-29 Dec 30 Probes Set] 30ng/ul x 80 ul, purified from 3 plates amplified using EconoTaq (20cycles).
*[http://genome-tech.ucsd.edu/LabNotes/index.php/Dinh/NOTES/2009-12-29 Dec 30 Probes Set] 30ng/ul x 80 ul, purified from 3 plates amplified using EconoTaq (20cycles).

Revision as of 02:41, 5 January 2010

Probes Preparation

  • Dec 30 Probes Set 30ng/ul x 80 ul, purified from 3 plates amplified using EconoTaq (20cycles).

Capturing

  • Improving capturing efficiency of CpG30K set for pipelining, Jan 5 2010 Capture Reaction (2pm),

<calendar> name=Dinh format=%name/NOTES/%year-%month-%day date=2010/07/01 view=oneyear </calendar>

<calendar> name=Dinh format=%name/NOTES/%year-%month-%day date=2009/07/01 view=oneyear </calendar>