Dinh:LAB/Bisulfite Sequencing: Difference between revisions

From ZhangLabWiki
Jump to navigation Jump to search
>Dinh
>Dinh
mNo edit summary
Line 12: Line 12:


*[http://genome-tech.ucsd.edu/LabNotes/index.php/Dinh/NOTES/2009-12-29 Dec 30 Probes Set] 30ng/ul x 80 ul, purified from 3 plates amplified using EconoTaq (20cycles).
*[http://genome-tech.ucsd.edu/LabNotes/index.php/Dinh/NOTES/2009-12-29 Dec 30 Probes Set] 30ng/ul x 80 ul, purified from 3 plates amplified using EconoTaq (20cycles).
=DNA Extraction=
* Practice on fibroblast cells culture in suspension [http://genome-tech.ucsd.edu/LabNotes/index.php/Dinh/NOTES/2009-12-1 AllPrep DNA/RNAeasy Kit]
* Cancer Patient Sample (1-9), [http://genome-tech.ucsd.edu/LabNotes/index.php/Dinh/NOTES/2009-12-8 DNeasy Blood & Tissue Kit]


=Bisulfite Conversion and Capturing=
=Bisulfite Conversion and Capturing=
Line 19: Line 23:
* Improving capturing efficiency of CpG30K set for pipelining, [http://genome-tech.ucsd.edu/LabNotes/index.php/Dinh/NOTES/2010-1-5 Jan 5 2010] Capture Reaction (2pm),  
* Improving capturing efficiency of CpG30K set for pipelining, [http://genome-tech.ucsd.edu/LabNotes/index.php/Dinh/NOTES/2010-1-5 Jan 5 2010] Capture Reaction (2pm),  


=Notebook=
<calendar>
<calendar>
name=Dinh
name=Dinh

Revision as of 02:45, 5 January 2010

Probes Preparation

  • Dec 30 Probes Set 30ng/ul x 80 ul, purified from 3 plates amplified using EconoTaq (20cycles).

DNA Extraction

Bisulfite Conversion and Capturing

  • Improving capturing efficiency of CpG30K set for pipelining, Jan 5 2010 Capture Reaction (2pm),

Notebook

<calendar> name=Dinh format=%name/NOTES/%year-%month-%day date=2010/07/01 view=oneyear </calendar>

<calendar> name=Dinh format=%name/NOTES/%year-%month-%day date=2009/07/01 view=oneyear </calendar>