AlanFung:LabNotes/Capturing/CPG97K Low-input/2009-11-25: Difference between revisions

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>Alan6017518
(New page: {| {{table}} | ||1well (ul)||X3||X1.1 |- | Template||10 |- | 2X Phusion||50||150||165 |- | AmpF6.2SoL (10uM)||4||12||13.2 |- | AmpR6.2Sol (10uM)||4||12||13.2 |- | 50X SYBG I||0.8||2.4||2.6...)
 
>Alan6017518
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==11/12/09==
{| {{table}}
{| {{table}}
| ||1well (ul)||X3||X1.1
| ||1well (ul)||X 8 ||X1.1  
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| Template||10
| Template||10||||
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| 2X Phusion||50||150||165
| 2X Phusion||50||400||440
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| AmpF6.2SoL (10uM)||4||12||13.2
| AmpF6.2SoL (10uM)||4||32||35.2
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| AmpR6.2Sol (10uM)||4||12||13.2
| AmpR6.2Sol (10uM)||4||32||35.2
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| 50X SYBG I||0.8||2.4||2.64
| 50X SYBG I||0.8||6.4||7.04
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| H2O||31.2||93.6||102.96
| H2O||31.2||249.6||274.56
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98C 30S -> (98C 10S -> 58C 20S -> 72C 20S) x 8 -> (98C 10S -> 72C 20S) x 20-> 72C 5 min -> 15C hold.

Latest revision as of 21:32, 25 November 2009

11/12/09[edit]

1well (ul) X 8 X1.1
Template 10
2X Phusion 50 400 440
AmpF6.2SoL (10uM) 4 32 35.2
AmpR6.2Sol (10uM) 4 32 35.2
50X SYBG I 0.8 6.4 7.04
H2O 31.2 249.6 274.56

98C 30S -> (98C 10S -> 58C 20S -> 72C 20S) x 8 -> (98C 10S -> 72C 20S) x 20-> 72C 5 min -> 15C hold.