AlanFung:LabNotes/Sequencing/2009-11-27: Difference between revisions
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>Alan6017518 |
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*aliquot 24ul to each well | *aliquot 24ul to each well | ||
98C 30 sec -> 20 cycles of (98C 10sec->64C 20sec->72C 20sec)->72C 5 min | 98C 30 sec -> 20 cycles of (98C 10sec->64C 20sec->72C 20sec)->72C 5 min | ||
{| {{table}} | |||
| ||||Sum||Ave||Diff||2^||X10 | |||
|- | |||
| CVF-N-S||8.162||24.091||8.030||2.819||7.057||70.567 | |||
|- | |||
| 0.070||8.040|||||||||| | |||
|- | |||
| ||7.889|||||||||| | |||
|- | |||
| PhiX Stock||11.020||32.548||10.849|||||| | |||
|- | |||
| 0.070||10.883|||||||||| | |||
|- | |||
| ||10.645|||||||||| | |||
|- | |||
| |||||||||||| | |||
|- | |||
| CVF-N A/B||7.850||32.161||8.040||1.791||3.460||34.599 | |||
|- | |||
| 0.050||8.002|||||||||| | |||
|- | |||
| ||8.146|||||||||| | |||
|- | |||
| ||8.163|||||||||| | |||
|- | |||
| PhiX Stock||9.665||29.493||9.831|||||| | |||
|- | |||
| 0.050||9.876|||||||||| | |||
|- | |||
| ||9.952|||||||||| | |||
|- | |||
| | |||
|} |
Latest revision as of 07:05, 27 November 2009
- Ethanol Precipitation
- Dissolve all in 14ul ddH2O
QPCR Quantification[edit]
Setup Stock Phi-X (1nM) CVF-N (Shearing) (1/10) Stock Phi-X (1nM) CVF-N (Shearing) (1/10) Stock Phi-X (1nM) CVF-N (Shearing) (1/10) Stock Phi-X (1nM) CVF-N (Shearing) (1/10) Amp Phi-X (1nM) CVF-N (A/B) (1/10) Amp Phi-X (1nM) CVF-N (A/B) (1/10) Amp Phi-X (1nM) CVF-N (A/B) (1/10) Amp Phi-X (1nM) CVF-N (A/B) (1/10)
X8 | X1.1 | ||
DNA | 1.000 | ||
Syb_FP5 (100uM) | 0.200 | 1.600 | 1.760 |
Syb_RP7 (100uM) | 0.200 | 1.600 | 1.760 |
2X Phusion | 25.000 | 200.000 | 220.000 |
50X SYBR Green I | 0.200 | 1.600 | 1.760 |
dH2O | 24.000 | 192.000 | 211.200 |
- Dilute sample, 1ul in 9ul ddh2o
- mix 2ul 1/10 sample with 98ul mix
- aliquot 24ul to each well
98C 30 sec -> 20 cycles of (98C 10sec->64C 20sec->72C 20sec)->72C 5 min
Sum | Ave | Diff | 2^ | X10 | ||
CVF-N-S | 8.162 | 24.091 | 8.030 | 2.819 | 7.057 | 70.567 |
0.070 | 8.040 | |||||
7.889 | ||||||
PhiX Stock | 11.020 | 32.548 | 10.849 | |||
0.070 | 10.883 | |||||
10.645 | ||||||
CVF-N A/B | 7.850 | 32.161 | 8.040 | 1.791 | 3.460 | 34.599 |
0.050 | 8.002 | |||||
8.146 | ||||||
8.163 | ||||||
PhiX Stock | 9.665 | 29.493 | 9.831 | |||
0.050 | 9.876 | |||||
9.952 | ||||||