AlanFung:LabNotes/Capturing/BisFirstExon 2009 12 10: Difference between revisions
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>Alan6017518 (New page: 1well (ul) X6 Template 10 2X iProof Mastermix 50 300 AmpF6.2SoL (10uM) 4 24 AmpR6.2Sol (10uM) 4 24 50X SYBG I 0.8 4.8 H2O 31.2 187.2 Total Volume 90.000 98C 30S -> (98C 10S -> 58C 20S ...) |
>Alan6017518 No edit summary |
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98C 30S -> (98C 10S -> 58C 20S -> 72C 20S) x 8 -> (98C 10S -> 72C 20S) x 20-> 72C 5 min -> 15C hold | 98C 30S -> (98C 10S -> 58C 20S -> 72C 20S) x 8 -> (98C 10S -> 72C 20S) x 20-> 72C 5 min -> 15C hold | ||
Setup | |||
NA12878 | |||
NA18507 | |||
NA20431 | |||
Jurkat New | |||
Jurkat Old |
Revision as of 21:08, 10 December 2009
1well (ul) X6 Template 10 2X iProof Mastermix 50 300 AmpF6.2SoL (10uM) 4 24 AmpR6.2Sol (10uM) 4 24 50X SYBG I 0.8 4.8 H2O 31.2 187.2 Total Volume 90.000
98C 30S -> (98C 10S -> 58C 20S -> 72C 20S) x 8 -> (98C 10S -> 72C 20S) x 20-> 72C 5 min -> 15C hold
Setup NA12878 NA18507 NA20431 Jurkat New Jurkat Old