AlanFung:LabNotes/Capturing/BisFirstExon 2009 12 10: Difference between revisions

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>Alan6017518
(New page: 1well (ul) X6 Template 10 2X iProof Mastermix 50 300 AmpF6.2SoL (10uM) 4 24 AmpR6.2Sol (10uM) 4 24 50X SYBG I 0.8 4.8 H2O 31.2 187.2 Total Volume 90.000 98C 30S -> (98C 10S -> 58C 20S ...)
 
>Alan6017518
 
(7 intermediate revisions by the same user not shown)
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1well (ul) X6
==PCR Amplification of Captured product==
Template 10
{| {{table}}
2X iProof Mastermix 50 300
| ||1well (ul)||X6
AmpF6.2SoL (10uM) 4 24
|-
AmpR6.2Sol (10uM) 4 24
| Template||10||
50X SYBG I 0.8 4.8
|-
H2O 31.2 187.2
| 2X iProof Mastermix||50||300
Total Volume 90.000
|-
| AmpF6.2SoL (10u0M)||0.4||2.4
|-
| AmpR6.2Sol (100uM)||0.4||2.4
|-
| 50X SYBG I||0.8||4.8
|-
| H2O||31.2||187.2
|-
| Total Volume||90.000||
|-
|
|}


98C 30S -> (98C 10S -> 58C 20S -> 72C 20S) x 8 -> (98C 10S -> 72C 20S) x 20-> 72C 5 min -> 15C hold
98C 30S -> (98C 10S -> 58C 20S -> 72C 20S) x 8 -> (98C 10S -> 72C 20S) x 20-> 72C 5 min -> 15C hold
{| {{table}}
| Setup
|-
| NA12878
|-
| NA18507
|-
| NA20431
|-
| Jurkat New
|-
| Jurkat Old
|-
|
|}
==TBE Gel Quantification==
[[Image:ZhangLab_2 2009-12-10 17hr 04min.jpg]]

Latest revision as of 01:14, 11 December 2009

PCR Amplification of Captured product[edit]

1well (ul) X6
Template 10
2X iProof Mastermix 50 300
AmpF6.2SoL (10u0M) 0.4 2.4
AmpR6.2Sol (100uM) 0.4 2.4
50X SYBG I 0.8 4.8
H2O 31.2 187.2
Total Volume 90.000

98C 30S -> (98C 10S -> 58C 20S -> 72C 20S) x 8 -> (98C 10S -> 72C 20S) x 20-> 72C 5 min -> 15C hold

Setup
NA12878
NA18507
NA20431
Jurkat New
Jurkat Old

TBE Gel Quantification[edit]

File:ZhangLab 2 2009-12-10 17hr 04min.jpg