AlanFung:LabNotes/Capturing/BisFirstExon 2009 12 10: Difference between revisions
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>Alan6017518 (New page: 1well (ul) X6 Template 10 2X iProof Mastermix 50 300 AmpF6.2SoL (10uM) 4 24 AmpR6.2Sol (10uM) 4 24 50X SYBG I 0.8 4.8 H2O 31.2 187.2 Total Volume 90.000 98C 30S -> (98C 10S -> 58C 20S ...) |
>Alan6017518 |
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==PCR Amplification of Captured product== | |||
Template 10 | {| {{table}} | ||
2X iProof Mastermix 50 300 | | ||1well (ul)||X6 | ||
AmpF6.2SoL ( | |- | ||
AmpR6.2Sol ( | | Template||10|| | ||
50X SYBG I 0.8 4.8 | |- | ||
H2O 31.2 187.2 | | 2X iProof Mastermix||50||300 | ||
Total Volume 90.000 | |- | ||
| AmpF6.2SoL (10u0M)||0.4||2.4 | |||
|- | |||
| AmpR6.2Sol (100uM)||0.4||2.4 | |||
|- | |||
| 50X SYBG I||0.8||4.8 | |||
|- | |||
| H2O||31.2||187.2 | |||
|- | |||
| Total Volume||90.000|| | |||
|- | |||
| | |||
|} | |||
98C 30S -> (98C 10S -> 58C 20S -> 72C 20S) x 8 -> (98C 10S -> 72C 20S) x 20-> 72C 5 min -> 15C hold | 98C 30S -> (98C 10S -> 58C 20S -> 72C 20S) x 8 -> (98C 10S -> 72C 20S) x 20-> 72C 5 min -> 15C hold | ||
{| {{table}} | |||
| Setup | |||
|- | |||
| NA12878 | |||
|- | |||
| NA18507 | |||
|- | |||
| NA20431 | |||
|- | |||
| Jurkat New | |||
|- | |||
| Jurkat Old | |||
|- | |||
| | |||
|} | |||
==TBE Gel Quantification== | |||
[[Image:ZhangLab_2 2009-12-10 17hr 04min.jpg]] |
Latest revision as of 01:14, 11 December 2009
PCR Amplification of Captured product[edit]
1well (ul) | X6 | |
Template | 10 | |
2X iProof Mastermix | 50 | 300 |
AmpF6.2SoL (10u0M) | 0.4 | 2.4 |
AmpR6.2Sol (100uM) | 0.4 | 2.4 |
50X SYBG I | 0.8 | 4.8 |
H2O | 31.2 | 187.2 |
Total Volume | 90.000 | |
98C 30S -> (98C 10S -> 58C 20S -> 72C 20S) x 8 -> (98C 10S -> 72C 20S) x 20-> 72C 5 min -> 15C hold
Setup |
NA12878 |
NA18507 |
NA20431 |
Jurkat New |
Jurkat Old |