AlanFung:LabNotes/Capturing/BisFirstExon 2009 12 10: Difference between revisions
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>Alan6017518 |
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==PCR Amplification of Captured product== | ==PCR Amplification of Captured product== | ||
{| {{table}} | |||
Template 10 | | ||1well (ul)||X6 | ||
2X iProof Mastermix 50 300 | |- | ||
AmpF6.2SoL ( | | Template||10|| | ||
AmpR6.2Sol ( | |- | ||
50X SYBG I 0.8 4.8 | | 2X iProof Mastermix||50||300 | ||
H2O 31.2 187.2 | |- | ||
Total Volume 90.000 | | AmpF6.2SoL (10u0M)||0.4||2.4 | ||
|- | |||
| AmpR6.2Sol (100uM)||0.4||2.4 | |||
|- | |||
| 50X SYBG I||0.8||4.8 | |||
|- | |||
| H2O||31.2||187.2 | |||
|- | |||
| Total Volume||90.000|| | |||
|- | |||
| | |||
|} | |||
98C 30S -> (98C 10S -> 58C 20S -> 72C 20S) x 8 -> (98C 10S -> 72C 20S) x 20-> 72C 5 min -> 15C hold | 98C 30S -> (98C 10S -> 58C 20S -> 72C 20S) x 8 -> (98C 10S -> 72C 20S) x 20-> 72C 5 min -> 15C hold | ||
{| {{table}} | {| {{table}} | ||
Line 35: | Line 39: | ||
==TBE Gel Quantification== | ==TBE Gel Quantification== | ||
[[Image:ZhangLab_2 2009-12-10 17hr 04min.jpg]] |
Latest revision as of 01:14, 11 December 2009
PCR Amplification of Captured product[edit]
1well (ul) | X6 | |
Template | 10 | |
2X iProof Mastermix | 50 | 300 |
AmpF6.2SoL (10u0M) | 0.4 | 2.4 |
AmpR6.2Sol (100uM) | 0.4 | 2.4 |
50X SYBG I | 0.8 | 4.8 |
H2O | 31.2 | 187.2 |
Total Volume | 90.000 | |
98C 30S -> (98C 10S -> 58C 20S -> 72C 20S) x 8 -> (98C 10S -> 72C 20S) x 20-> 72C 5 min -> 15C hold
Setup |
NA12878 |
NA18507 |
NA20431 |
Jurkat New |
Jurkat Old |