AlanFung:LabNotes/Capturing/BisFirstExon 2009 12 10: Difference between revisions
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>Alan6017518 |
>Alan6017518 |
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| 2X iProof Mastermix||50||300 | | 2X iProof Mastermix||50||300 | ||
|- | |- | ||
| AmpF6.2SoL ( | | AmpF6.2SoL (10u0M)||0.4||2.4 | ||
|- | |- | ||
| AmpR6.2Sol ( | | AmpR6.2Sol (100uM)||0.4||2.4 | ||
|- | |- | ||
| 50X SYBG I||0.8||4.8 | | 50X SYBG I||0.8||4.8 | ||
Line 39: | Line 39: | ||
==TBE Gel Quantification== | ==TBE Gel Quantification== | ||
[[Image:ZhangLab_2 2009-12-10 17hr 04min.jpg]] |
Latest revision as of 01:14, 11 December 2009
PCR Amplification of Captured product[edit]
1well (ul) | X6 | |
Template | 10 | |
2X iProof Mastermix | 50 | 300 |
AmpF6.2SoL (10u0M) | 0.4 | 2.4 |
AmpR6.2Sol (100uM) | 0.4 | 2.4 |
50X SYBG I | 0.8 | 4.8 |
H2O | 31.2 | 187.2 |
Total Volume | 90.000 | |
98C 30S -> (98C 10S -> 58C 20S -> 72C 20S) x 8 -> (98C 10S -> 72C 20S) x 20-> 72C 5 min -> 15C hold
Setup |
NA12878 |
NA18507 |
NA20431 |
Jurkat New |
Jurkat Old |