AlanFung:LabNotes/Capturing/BisFirstExon 2009 12 31: Difference between revisions

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>Alan6017518
 
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{| {{table}}
{| {{table}}
| ||1 well (uL)||X 6||||||1 well (uL)||X 6
| ||1 well (uL)||X 6
|-
|-
| Template||10||||||Template||||
| Template||10||
|-
|-
| 2X iProof Mastermix||50||300||||2X iProof Mastermix||50||300
| 2X iProof Mastermix||50||300
|-
|-
| Amp F6.3 Sol (100uM)||0.4||2.4||||Amp F6.3r Sol (100uM)||0.4||2.4
| Amp F6.3 Sol (100uM)||0.4||2.4
|-
|-
| Amp R6.3Sol (100uM)||0.4||2.4||||Amp R6.3r Sol (100uM)||0.4||2.4
| Amp R6.3Sol (100uM)||0.4||2.4
|-
|-
| 50X SYBG I||0.8||4.8||||50X SYBG I||0.8||4.8
| 50X SYBG I||0.8||4.8
|-
|-
| H2O||31.2||187.2||||H2O||31.2||187.2
| H2O||31.2||187.2
|-
|-
| Total Volume||90||540||||Total Volume||90||540
| Total Volume||90||540
|-
|-
|  
|  

Latest revision as of 20:44, 4 January 2010

PCR Amplification of Captured product[edit]

NA12878 NA18507 NA20431 Jurkat New Jurkat Old[edit]

1 well (uL) X 6
Template 10
2X iProof Mastermix 50 300
Amp F6.3 Sol (100uM) 0.4 2.4
Amp R6.3Sol (100uM) 0.4 2.4
50X SYBG I 0.8 4.8
H2O 31.2 187.2
Total Volume 90 540

98C 30S -> (98C 10S -> 60C 20S -> 72C 20S) x 8 -> (98C 10S -> 72C 20S) x 20-> 72C 5 min -> 15C hold

TBE Gel[edit]

File:ZhangLab 2 2010-01-04 12hr 26min.jpg