AlanFung:LabNotes/Capturing/BisFirstExon 2009 12 31: Difference between revisions
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{| {{table}} | {| {{table}} | ||
| ||1 well (uL)||X 6 | |||
|- | |- | ||
| Template||10 | | Template||10|| | ||
|- | |- | ||
| 2X iProof Mastermix||50||300 | |||
|- | |- | ||
| Amp F6.3 | | Amp F6.3 Sol (100uM)||0.4||2.4 | ||
|- | |- | ||
| Amp R6.3Sol | | Amp R6.3Sol (100uM)||0.4||2.4 | ||
|- | |- | ||
| 50X SYBG I||0.8||4.8 | |||
|- | |- | ||
| H2O||31.2||187.2 | |||
|- | |- | ||
| Total Volume||90||540 | |||
|- | |- | ||
| | | |
Latest revision as of 20:44, 4 January 2010
PCR Amplification of Captured product[edit]
NA12878 NA18507 NA20431 Jurkat New Jurkat Old[edit]
1 well (uL) | X 6 | |
Template | 10 | |
2X iProof Mastermix | 50 | 300 |
Amp F6.3 Sol (100uM) | 0.4 | 2.4 |
Amp R6.3Sol (100uM) | 0.4 | 2.4 |
50X SYBG I | 0.8 | 4.8 |
H2O | 31.2 | 187.2 |
Total Volume | 90 | 540 |
98C 30S -> (98C 10S -> 60C 20S -> 72C 20S) x 8 -> (98C 10S -> 72C 20S) x 20-> 72C 5 min -> 15C hold