Illumina GA/Oligo info: Difference between revisions
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| Syb_RP7 ||Sol. Quant. (Reward)||CAAGCAGAAGACGGCATACGAG||None||[2] | | Syb_RP7 ||Sol. Quant. (Reward)||CAAGCAGAAGACGGCATACGAG||None||[2] | ||
| | |||
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| | | PE_t_N2||Y-adaptor for MmeI ligation (top)||ACACTCTTTCCCTACACGACGCTCTTCCGATCTN*N||3'-Phosphorothioate bond|| | ||
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| PE_N2||Y-adaptor for MmeI ligation (top)||ACACTCTTTCCCTACACGACGCTCTTCCGATC*NN||3'-Phosphorothioate bond|| | |||
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| PE_b_A||Y-adaptor for MmeI ligation (bottom)||\\5phos\\AGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG||5'-phosphorylation|| | |||
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| PE_t_Met||bis-seq(Forward)||A/iMe-dC/A/iMe-dC/T/iMe-dC/TTT/iMe-dC//iMe-dC//iMe-dC/TA/iMe-dC/ACGACG/iMe-dC/T/iMe-dC/TT/iMe-dC/CGAT/iMe-dC/*T||3-phosphorothiate, 5'-Methyl|| | | PE_t_Met||bis-seq(Forward)||A/iMe-dC/A/iMe-dC/T/iMe-dC/TTT/iMe-dC//iMe-dC//iMe-dC/TA/iMe-dC/ACGACG/iMe-dC/T/iMe-dC/TT/iMe-dC/CGAT/iMe-dC/*T||3-phosphorothiate, 5'-Methyl|| | ||
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| PE_b_Met||bis-seq (Reward)||GATCGGAAGAGCGGTT/iMe-dC/AG/iMe-dC/AGGAATG/iMe-dC/CGAG||5'-phosphorylated, 5'-Methyl|| | | PE_b_Met||bis-seq (Reward)||/5Phos/GATCGGAAGAGCGGTT/iMe-dC/AG/iMe-dC/AGGAATG/iMe-dC/CGAG||5'-phosphorylated, 5'-Methyl|| | ||
|} | |} | ||
Revision as of 19:34, 23 January 2010
Name in original paper | Nick-name | Sequence (5\'->3\') | Sequence Modification | Reference | |
A_adapter_t | Long Y-adaptor (top) | AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATC*T | 3'-Phosphorothioate bond | [1] | |
A_adapter_b | Long Y-adaptor (bottom) | GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTG | None | [1] | |
PE_t_adapter | Regular Y-adaptor (top) | ACACTCTTTCCCTACACGACGCTCTTCCGATC*T | 3'-Phosphorothioate bond | [2] | |
PE_b_adapter | Regular Y-adaptor (bottom) | \\5phos\\GATCGGAAGAGCGGTTCAGCAGGAATGCCGAG | 5'-phosphorylation | [2] | |
PCR_F | Sol. Amp. (Forward) | AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATC*T | 3'-Phosphorothioate bond | [2] | |
PCR_R | Sol. Amp. (Reward) | CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATC*T | 3'-Phosphorothioate bond | [2] | |
Syb_FP5 | Sol. Quant. (Forward) | ATGATACGGCGACCACCGAG | None | [2] | |
Syb_RP7 | Sol. Quant. (Reward) | CAAGCAGAAGACGGCATACGAG | None | [2] | |
PE_t_N2 | Y-adaptor for MmeI ligation (top) | ACACTCTTTCCCTACACGACGCTCTTCCGATCTN*N | 3'-Phosphorothioate bond | ||
PE_N2 | Y-adaptor for MmeI ligation (top) | ACACTCTTTCCCTACACGACGCTCTTCCGATC*NN | 3'-Phosphorothioate bond | ||
PE_b_A | Y-adaptor for MmeI ligation (bottom) | \\5phos\\AGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG | 5'-phosphorylation | ||
PE_t_Met | bis-seq(Forward) | A/iMe-dC/A/iMe-dC/T/iMe-dC/TTT/iMe-dC//iMe-dC//iMe-dC/TA/iMe-dC/ACGACG/iMe-dC/T/iMe-dC/TT/iMe-dC/CGAT/iMe-dC/*T | 3-phosphorothiate, 5'-Methyl | ||
PE_b_Met | bis-seq (Reward) | /5Phos/GATCGGAAGAGCGGTT/iMe-dC/AG/iMe-dC/AGGAATG/iMe-dC/CGAG | 5'-phosphorylated, 5'-Methyl |