Illumina GA/Oligo info: Difference between revisions
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| PCR_F||Sol. Amp. (Forward)||AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATC*T||3'-Phosphorothioate bond||[2] | | PCR_F||Sol. Amp. (Forward)||AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATC*T||3'-Phosphorothioate bond||[2] | ||
|- | |- | ||
| PCR_R||Sol. Amp. ( | | PCR_R||Sol. Amp. (Reverse)||CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATC*T||3'-Phosphorothioate bond||[2] | ||
|- | |- | ||
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| Syb_FP5||Sol. Quant. (Forward)||ATGATACGGCGACCACCGAG||None||[2] | | Syb_FP5||Sol. Quant. (Forward)||ATGATACGGCGACCACCGAG||None||[2] | ||
|- | |- | ||
| Syb_RP7 ||Sol. Quant. ( | | Syb_RP7 ||Sol. Quant. (Reverse)||CAAGCAGAAGACGGCATACGAG||None||[2] | ||
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| PE_t_Met||bis-seq(Forward)||A/iMe-dC/A/iMe-dC/T/iMe-dC/TTT/iMe-dC//iMe-dC//iMe-dC/TA/iMe-dC/ACGACG/iMe-dC/T/iMe-dC/TT/iMe-dC/CGAT/iMe-dC/*T||3-phosphorothiate, 5'-Methyl|| | | PE_t_Met||bis-seq(Forward)||A/iMe-dC/A/iMe-dC/T/iMe-dC/TTT/iMe-dC//iMe-dC//iMe-dC/TA/iMe-dC/ACGACG/iMe-dC/T/iMe-dC/TT/iMe-dC/CGAT/iMe-dC/*T||3-phosphorothiate, 5'-Methyl|| | ||
|- | |- | ||
| PE_b_Met||bis-seq ( | | PE_b_Met||bis-seq (Reverse)||/5Phos/GATCGGAAGAGCGGTT/iMe-dC/AG/iMe-dC/AGGAATG/iMe-dC/CGAG||5'-phosphorylated, 5'-Methyl|| | ||
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**[[Media:Amplification-free Illumina sequencing-library preparation facilatates improved mapping and assembly of GC biased genomes-supp.pdf|[1] supp]] | **[[Media:Amplification-free Illumina sequencing-library preparation facilatates improved mapping and assembly of GC biased genomes-supp.pdf|[1] supp]] | ||
*[[Media:Improved protocols for the Illumina GA.pdf|[2] Improved protocols for the Illumina GA]] | *[[Media:Improved protocols for the Illumina GA.pdf|[2] Improved protocols for the Illumina GA]] | ||
=Multiplexing Primers= | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Name''' | |||
| align="center" style="background:#f0f0f0;"|'''Nick-name''' | |||
| align="center" style="background:#f0f0f0;"|'''Sequence (5\'->3\')''' | |||
| align="center" style="background:#f0f0f0;"|'''Sequence Modification''' | |||
| align="center" style="background:#f0f0f0;"|'''Reference''' | |||
|- | |||
| Left Probe Linker, Barcode 1: CGTGAT || AmpR6.3Ind1 || ||None||None | |||
|- | |||
| Left Probe Linker, Barcode 2: ACATCG || AmpR6.3Ind2 || ||None||None | |||
|- | |||
| Left Probe Linker, Barcode 3: GCCTAA || AmpR6.3Ind3 || ||None||None | |||
|- | |||
| Left Probe Linker, Barcode 4: TGGTCA || AmpR6.3Ind4 || ||None||None | |||
|- | |||
| Left Probe Linker, Barcode 5: CACTGT || AmpR6.3Ind5 || ||None||None | |||
|- | |||
| Left Probe Linker, Barcode 6: ATTGGC || AmpR6.3Ind6 || ||None||None | |||
|- | |||
| Left Probe Linker, Barcode 7: GATCTG || AmpR6.3Ind7 || ||None||None | |||
|- | |||
| Left Probe Linker, Barcode 8: TCAAGT || AmpR6.3Ind8 || ||None||None | |||
|- | |||
| Left Probe Linker, Barcode 9: CTGATC || AmpR6.3Ind9 || ||None||None | |||
|- | |||
| Left Probe Linker, Barcode 10: AAGCTA || AmpR6.3Ind10 || ||None||None | |||
|- | |||
| Left Probe Linker, Barcode 11: GTAGCC || AmpR6.3Ind11 || ||None||None | |||
|- | |||
| Left Probe Linker, Barcode 12: TACAAG || AmpR6.3Ind12 || ||None||None | |||
|- | |||
|} |
Revision as of 21:24, 15 July 2010
Name in original paper | Nick-name | Sequence (5\'->3\') | Sequence Modification | Reference | |
A_adapter_t | Long Y-adaptor (top) | AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATC*T | 3'-Phosphorothioate bond | [1] | |
A_adapter_b | Long Y-adaptor (bottom) | GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTG | None | [1] | |
PE_t_adapter | Regular Y-adaptor (top) | ACACTCTTTCCCTACACGACGCTCTTCCGATC*T | 3'-Phosphorothioate bond | [2] | |
PE_b_adapter | Regular Y-adaptor (bottom) | /5Phos/GATCGGAAGAGCGGTTCAGCAGGAATGCCGAG | 5'-phosphorylation | [2] | |
PCR_F | Sol. Amp. (Forward) | AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATC*T | 3'-Phosphorothioate bond | [2] | |
PCR_R | Sol. Amp. (Reverse) | CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATC*T | 3'-Phosphorothioate bond | [2] | |
Syb_FP5 | Sol. Quant. (Forward) | ATGATACGGCGACCACCGAG | None | [2] | |
Syb_RP7 | Sol. Quant. (Reverse) | CAAGCAGAAGACGGCATACGAG | None | [2] | |
PE_t_N2 | Y-adaptor for MmeI ligation (top) | ACACTCTTTCCCTACACGACGCTCTTCCGATCTN*N | 3'-Phosphorothioate bond | ||
PE_N2 | Y-adaptor for MmeI ligation (top) | ACACTCTTTCCCTACACGACGCTCTTCCGATC*NN | 3'-Phosphorothioate bond | ||
PE_b_A | Y-adaptor for MmeI ligation (bottom) | /5Phos/AGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG | 5'-phosphorylation | ||
PE_t_Met | bis-seq(Forward) | A/iMe-dC/A/iMe-dC/T/iMe-dC/TTT/iMe-dC//iMe-dC//iMe-dC/TA/iMe-dC/ACGACG/iMe-dC/T/iMe-dC/TT/iMe-dC/CGAT/iMe-dC/*T | 3-phosphorothiate, 5'-Methyl | ||
PE_b_Met | bis-seq (Reverse) | /5Phos/GATCGGAAGAGCGGTT/iMe-dC/AG/iMe-dC/AGGAATG/iMe-dC/CGAG | 5'-phosphorylated, 5'-Methyl | ||
RC_PE_t_adapter | RC_Regular Y-adaptor with Biotin (top) | /5Biosg/TTTTTTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT | 5'-Biotin | ||
RC_PE_b_adapter | RC_Regular Y-adaptor with Biotin (bottom) | /5Biosg/TTTTTTTCTCGGCATTCCTGCTGAACCGCTCTTCCGATC | 5'-Biotin | ||
Small RNA Sequencing Primer | Solexa-TSS-Seq | CGACAGGTTCAGAGTTCTACAGTCCGACGATC | |||
- [1] Amplification-free Illumina sequencing-library preparation facilatates improved mapping and assembly of GC biased genomes
- [2] Improved protocols for the Illumina GA
Multiplexing Primers
Name | Nick-name | Sequence (5\'->3\') | Sequence Modification | Reference |
Left Probe Linker, Barcode 1: CGTGAT | AmpR6.3Ind1 | None | None | |
Left Probe Linker, Barcode 2: ACATCG | AmpR6.3Ind2 | None | None | |
Left Probe Linker, Barcode 3: GCCTAA | AmpR6.3Ind3 | None | None | |
Left Probe Linker, Barcode 4: TGGTCA | AmpR6.3Ind4 | None | None | |
Left Probe Linker, Barcode 5: CACTGT | AmpR6.3Ind5 | None | None | |
Left Probe Linker, Barcode 6: ATTGGC | AmpR6.3Ind6 | None | None | |
Left Probe Linker, Barcode 7: GATCTG | AmpR6.3Ind7 | None | None | |
Left Probe Linker, Barcode 8: TCAAGT | AmpR6.3Ind8 | None | None | |
Left Probe Linker, Barcode 9: CTGATC | AmpR6.3Ind9 | None | None | |
Left Probe Linker, Barcode 10: AAGCTA | AmpR6.3Ind10 | None | None | |
Left Probe Linker, Barcode 11: GTAGCC | AmpR6.3Ind11 | None | None | |
Left Probe Linker, Barcode 12: TACAAG | AmpR6.3Ind12 | None | None |