Illumina GA/Oligo info: Difference between revisions
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| align="center" style="background:#f0f0f0;"|'''Reference''' | | align="center" style="background:#f0f0f0;"|'''Reference''' | ||
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| Left Probe Linker, No barcode (forward) || AmpF6.3Sol || ||None||None | | Left Probe Linker, No barcode (forward) || AmpF6.3Sol || AATGATACGGCGACCACCGACACTCTCAGATGTTATCGAGGTCCGA ||None||None | ||
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| Right Probe Linker, Barcode 1: '''CGTGAT''' (reverse)|| AmpR6.3Ind1 || | | Right Probe Linker, Barcode 1: '''CGTGAT''' (reverse)|| AmpR6.3Ind1 || CAAGCAGAAGACGGCATACGAGATCGTGATGCTAGGAACGATGAGCCTCCAAC ||None||None | ||
|- | |- | ||
| Right Probe Linker, Barcode 2: '''ACATCG''' (reverse)|| AmpR6.3Ind2 || ||None||None | | Right Probe Linker, Barcode 2: '''ACATCG''' (reverse)|| AmpR6.3Ind2 || CAAGCAGAAGACGGCATACGAGATACATCGGCTAGGAACGATGAGCCTCCAAC ||None||None | ||
|- | |- | ||
| Right Probe Linker, Barcode 3: '''GCCTAA''' (reverse)|| AmpR6.3Ind3 || ||None||None | | Right Probe Linker, Barcode 3: '''GCCTAA''' (reverse)|| AmpR6.3Ind3 || CAAGCAGAAGACGGCATACGAGATGCCTAAGCTAGGAACGATGAGCCTCCAAC ||None||None | ||
|- | |||
| Right Probe Linker, Barcode 4: '''TGGTCA''' (reverse)|| AmpR6.3Ind4 || CAAGCAGAAGACGGCATACGAGATTGGTCAGCTAGGAACGATGAGCCTCCAAC ||None||None | |||
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| Right Probe Linker, Barcode | | Right Probe Linker, Barcode 5: '''CACTGT''' (reverse)|| AmpR6.3Ind5 || CAAGCAGAAGACGGCATACGAGATCACTGTGCTAGGAACGATGAGCCTCCAAC ||None||None | ||
|- | |||
| Right Probe Linker, Barcode 6: '''ATTGGC''' (reverse)|| AmpR6.3Ind6 || CAAGCAGAAGACGGCATACGAGATATTGGCGCTAGGAACGATGAGCCTCCAAC ||None||None | |||
|- | |- | ||
| Right Probe Linker, Barcode | | Right Probe Linker, Barcode 7: '''GATCTG''' (reverse)|| AmpR6.3Ind7 || CAAGCAGAAGACGGCATACGAGATGATCTGGCTAGGAACGATGAGCCTCCAAC ||None||None | ||
|- | |- | ||
| Right Probe Linker, Barcode | | Right Probe Linker, Barcode 8: '''TCAAGT''' (reverse)|| AmpR6.3Ind8 || CAAGCAGAAGACGGCATACGAGATTCAAGTGCTAGGAACGATGAGCCTCCAAC ||None||None | ||
|- | |- | ||
| Right Probe Linker, Barcode | | Right Probe Linker, Barcode 9: '''CTGATC''' (reverse)|| AmpR6.3Ind9 || CAAGCAGAAGACGGCATACGAGATCTGATCGCTAGGAACGATGAGCCTCCAAC ||None||None | ||
|- | |- | ||
| Right Probe Linker, Barcode | | Right Probe Linker, Barcode 10: '''AAGCTA''' (reverse)|| AmpR6.3Ind10 || CAAGCAGAAGACGGCATACGAGATAAGCTAGCTAGGAACGATGAGCCTCCAAC ||None||None | ||
|- | |- | ||
| Right Probe Linker, Barcode | | Right Probe Linker, Barcode 11: '''GTAGCC''' (reverse)|| AmpR6.3Ind11 || CAAGCAGAAGACGGCATACGAGATGTAGCCGCTAGGAACGATGAGCCTCCAAC ||None||None | ||
|- | |- | ||
| Right Probe Linker, Barcode 12: '''TACAAG''' (reverse)|| AmpR6.3Ind12 || CAAGCAGAAGACGGCATACGAGATTACAAGGCTAGGAACGATGAGCCTCCAAC ||None||None | |||
| Right Probe Linker, Barcode 12: '''TACAAG''' (reverse)|| AmpR6.3Ind12 || ||None||None | |||
|- | |- | ||
|} | |} |
Revision as of 21:44, 15 July 2010
Name in original paper | Nick-name | Sequence (5\'->3\') | Sequence Modification | Reference | |
A_adapter_t | Long Y-adaptor (top) | AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATC*T | 3'-Phosphorothioate bond | [1] | |
A_adapter_b | Long Y-adaptor (bottom) | GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTG | None | [1] | |
PE_t_adapter | Regular Y-adaptor (top) | ACACTCTTTCCCTACACGACGCTCTTCCGATC*T | 3'-Phosphorothioate bond | [2] | |
PE_b_adapter | Regular Y-adaptor (bottom) | /5Phos/GATCGGAAGAGCGGTTCAGCAGGAATGCCGAG | 5'-phosphorylation | [2] | |
PCR_F | Sol. Amp. (Forward) | AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATC*T | 3'-Phosphorothioate bond | [2] | |
PCR_R | Sol. Amp. (Reverse) | CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATC*T | 3'-Phosphorothioate bond | [2] | |
Syb_FP5 | Sol. Quant. (Forward) | ATGATACGGCGACCACCGAG | None | [2] | |
Syb_RP7 | Sol. Quant. (Reverse) | CAAGCAGAAGACGGCATACGAG | None | [2] | |
PE_t_N2 | Y-adaptor for MmeI ligation (top) | ACACTCTTTCCCTACACGACGCTCTTCCGATCTN*N | 3'-Phosphorothioate bond | ||
PE_N2 | Y-adaptor for MmeI ligation (top) | ACACTCTTTCCCTACACGACGCTCTTCCGATC*NN | 3'-Phosphorothioate bond | ||
PE_b_A | Y-adaptor for MmeI ligation (bottom) | /5Phos/AGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG | 5'-phosphorylation | ||
PE_t_Met | bis-seq(Forward) | A/iMe-dC/A/iMe-dC/T/iMe-dC/TTT/iMe-dC//iMe-dC//iMe-dC/TA/iMe-dC/ACGACG/iMe-dC/T/iMe-dC/TT/iMe-dC/CGAT/iMe-dC/*T | 3-phosphorothiate, 5'-Methyl | ||
PE_b_Met | bis-seq (Reverse) | /5Phos/GATCGGAAGAGCGGTT/iMe-dC/AG/iMe-dC/AGGAATG/iMe-dC/CGAG | 5'-phosphorylated, 5'-Methyl | ||
RC_PE_t_adapter | RC_Regular Y-adaptor with Biotin (top) | /5Biosg/TTTTTTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT | 5'-Biotin | ||
RC_PE_b_adapter | RC_Regular Y-adaptor with Biotin (bottom) | /5Biosg/TTTTTTTCTCGGCATTCCTGCTGAACCGCTCTTCCGATC | 5'-Biotin | ||
Small RNA Sequencing Primer | Solexa-TSS-Seq | CGACAGGTTCAGAGTTCTACAGTCCGACGATC | |||
- [1] Amplification-free Illumina sequencing-library preparation facilatates improved mapping and assembly of GC biased genomes
- [2] Improved protocols for the Illumina GA
Multiplexing Primers
Name | Nick-name | Sequence (5\'->3\') | Sequence Modification | Reference |
Left Probe Linker, No barcode (forward) | AmpF6.3Sol | AATGATACGGCGACCACCGACACTCTCAGATGTTATCGAGGTCCGA | None | None |
Right Probe Linker, Barcode 1: CGTGAT (reverse) | AmpR6.3Ind1 | CAAGCAGAAGACGGCATACGAGATCGTGATGCTAGGAACGATGAGCCTCCAAC | None | None |
Right Probe Linker, Barcode 2: ACATCG (reverse) | AmpR6.3Ind2 | CAAGCAGAAGACGGCATACGAGATACATCGGCTAGGAACGATGAGCCTCCAAC | None | None |
Right Probe Linker, Barcode 3: GCCTAA (reverse) | AmpR6.3Ind3 | CAAGCAGAAGACGGCATACGAGATGCCTAAGCTAGGAACGATGAGCCTCCAAC | None | None |
Right Probe Linker, Barcode 4: TGGTCA (reverse) | AmpR6.3Ind4 | CAAGCAGAAGACGGCATACGAGATTGGTCAGCTAGGAACGATGAGCCTCCAAC | None | None |
Right Probe Linker, Barcode 5: CACTGT (reverse) | AmpR6.3Ind5 | CAAGCAGAAGACGGCATACGAGATCACTGTGCTAGGAACGATGAGCCTCCAAC | None | None |
Right Probe Linker, Barcode 6: ATTGGC (reverse) | AmpR6.3Ind6 | CAAGCAGAAGACGGCATACGAGATATTGGCGCTAGGAACGATGAGCCTCCAAC | None | None |
Right Probe Linker, Barcode 7: GATCTG (reverse) | AmpR6.3Ind7 | CAAGCAGAAGACGGCATACGAGATGATCTGGCTAGGAACGATGAGCCTCCAAC | None | None |
Right Probe Linker, Barcode 8: TCAAGT (reverse) | AmpR6.3Ind8 | CAAGCAGAAGACGGCATACGAGATTCAAGTGCTAGGAACGATGAGCCTCCAAC | None | None |
Right Probe Linker, Barcode 9: CTGATC (reverse) | AmpR6.3Ind9 | CAAGCAGAAGACGGCATACGAGATCTGATCGCTAGGAACGATGAGCCTCCAAC | None | None |
Right Probe Linker, Barcode 10: AAGCTA (reverse) | AmpR6.3Ind10 | CAAGCAGAAGACGGCATACGAGATAAGCTAGCTAGGAACGATGAGCCTCCAAC | None | None |
Right Probe Linker, Barcode 11: GTAGCC (reverse) | AmpR6.3Ind11 | CAAGCAGAAGACGGCATACGAGATGTAGCCGCTAGGAACGATGAGCCTCCAAC | None | None |
Right Probe Linker, Barcode 12: TACAAG (reverse) | AmpR6.3Ind12 | CAAGCAGAAGACGGCATACGAGATTACAAGGCTAGGAACGATGAGCCTCCAAC | None | None |