Capture Test of CES36k18bp: Difference between revisions

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Probe:
Probe:
  CES36k18bp: 5.6ng/ul;36,000 probes; 170nM
  CES36k18bp: 5.6ng/ul;36,000 probes; 170nM
  Volume of CES36k18bp needed =
  Volume of CES36k18bp needed = (200ng/3pg * 100 * 36000)/(6.0 * 10^23 * 170 nM) = 1.73ul

Revision as of 23:12, 5 January 2010

Capture Protocol

Probe and Sample

Probe is CES36k18bp, 5.6ng/ul Sample is Jurkat DNA standard, 100ng/ul

The Probe:Target ratio is 100:1

Calculations

Probe:

CES36k18bp: 5.6ng/ul;36,000 probes; 170nM
Volume of CES36k18bp needed = (200ng/3pg * 100 * 36000)/(6.0 * 10^23 * 170 nM) = 1.73ul