Capture Test of CES36k18bp: Difference between revisions

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'''Probe and Sample'''
'''Probe and Sample'''


Probe is CES36k18bp, 5.6ng/ul
Probes: CES36k18bp, 5.6ng/ul
       
Sample is Jurkat DNA standard, 100ng/ul
Sample is Jurkat DNA standard, 100ng/ul


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Probe:
Probe:
  CES36k18bp: 5.6ng/ul;36,000 probes; 170nM
  CES36k18bp: 5.6ng/ul;36,000 probes; 170nM
  Volume of CES36k18bp needed = (200ng/3pg * 100 * 36000)/(6.0 * 10^23 * 170 nM) = 1.73ul
  Volume of CES36k18bp needed = (200ng/3pg * 100 * 36000)/(6.0 * 10^23 * 170 nM) = 2.35ul
 
'''Reaction Setup'''

Revision as of 23:18, 5 January 2010

Capture Protocol

Probe and Sample

Probes: CES36k18bp, 5.6ng/ul

Sample is Jurkat DNA standard, 100ng/ul

The Probe:Target ratio is 100:1

Calculations

Probe:

CES36k18bp: 5.6ng/ul;36,000 probes; 170nM
Volume of CES36k18bp needed = (200ng/3pg * 100 * 36000)/(6.0 * 10^23 * 170 nM) = 2.35ul

Reaction Setup