Capture Test of CES36k18bp: Difference between revisions
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'''Probe and Sample''' | '''Probe and Sample''' | ||
Probes: CES36k18bp, 5.6ng/ul | |||
Sample is Jurkat DNA standard, 100ng/ul | Sample is Jurkat DNA standard, 100ng/ul | ||
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Probe: | Probe: | ||
CES36k18bp: 5.6ng/ul;36,000 probes; 170nM | CES36k18bp: 5.6ng/ul;36,000 probes; 170nM | ||
Volume of CES36k18bp needed = (200ng/3pg * 100 * 36000)/(6.0 * 10^23 * 170 nM) = | Volume of CES36k18bp needed = (200ng/3pg * 100 * 36000)/(6.0 * 10^23 * 170 nM) = 2.35ul | ||
'''Reaction Setup''' |
Revision as of 23:18, 5 January 2010
Capture Protocol
Probe and Sample
Probes: CES36k18bp, 5.6ng/ul
Sample is Jurkat DNA standard, 100ng/ul
The Probe:Target ratio is 100:1
Calculations
Probe:
CES36k18bp: 5.6ng/ul;36,000 probes; 170nM Volume of CES36k18bp needed = (200ng/3pg * 100 * 36000)/(6.0 * 10^23 * 170 nM) = 2.35ul
Reaction Setup