Calendar/2010-1-16: Difference between revisions
Jump to navigation
Jump to search
>Alan6017518 No edit summary |
>Alan6017518 |
||
Line 51: | Line 51: | ||
[[Media:AL-Exome-CViB-Jan14-AB.pdf]] | [[Media:AL-Exome-CViB-Jan14-AB.pdf]] | ||
[[Media:AL-Exome-CViB-Jan14-CDE.pdf]] | [[Media:AL-Exome-CViB-Jan14-CDE.pdf]] | ||
{| {{table}} | |||
| ||Well / Set||C(t)||||Difference||nM | |||
|- | |||
| PhiX||A1||6.98||7.06|||| | |||
|- | |||
| PhiX||B1||7.2|||||| | |||
|- | |||
| PhiX||C1||7.16|||||| | |||
|- | |||
| PhiX||D1||6.89|||||| | |||
|- | |||
| JD-cpg97k+30k-Breast_cancer-01230B09-Jan15||E1||6.82||6.92||0.14||1.1 | |||
|- | |||
| JD-cpg97k+30k-Breast_cancer-01230B09-Jan15||F1||7.09|||||| | |||
|- | |||
| JD-cpg97k+30k-Breast_cancer-01230B09-Jan15||G1||6.89|||||| | |||
|- | |||
| JD-cpg97k+30k-Breast_cancer-01230B09-Jan15||H1||6.86|||||| | |||
|- | |||
| | |||
|} |
Revision as of 18:17, 21 January 2010
Quantify libraries with Phi-X ladder qPCR
AL-Exome-CViB-Jan14-AB w/ 1/10 dilution, AL-Exome-CViB-Jan14-CDE w/ 1/10 dilution, Ida's library
Setup | ||
1nm PhiX | AL-Exome-CViB-Jan14-AB | AL-Exome-CViB-Jan14-CDE |
1nm PhiX | AL-Exome-CViB-Jan14-AB | AL-Exome-CViB-Jan14-CDE |
1nm PhiX | AL-Exome-CViB-Jan14-AB | AL-Exome-CViB-Jan14-CDE |
1nm PhiX | AL-Exome-CViB-Jan14-AB | AL-Exome-CViB-Jan14-CDE |
Ida | 1/10 AL-Exome-CViB-Jan14-AB | 1/10 AL-Exome-CViB-Jan14-CDE |
Ida | 1/10 AL-Exome-CViB-Jan14-AB | 1/10 AL-Exome-CViB-Jan14-CDE |
Ida | 1/10 AL-Exome-CViB-Jan14-AB | 1/10 AL-Exome-CViB-Jan14-CDE |
Ida | 1/10 AL-Exome-CViB-Jan14-AB | 1/10 AL-Exome-CViB-Jan14-CDE |
2 Wells | X13 | |
DNA | 2 | 26 |
Syb_FP5 (100uM) | 0.2 | 2.6 |
Syb_RP7 (100uM) | 0.2 | 2.6 |
2X Phusion | 25 | , |
50X SYBR Green | 0.2 | 2.6 |
H2O | 24 | 312 |
Total | 345.8 | |
* Dilute sample, 1ul in 9ul ddh2o * mix 2ul 1/10 sample with 98ul mix in non-stick tube * aliquot 24ul to each well, for each sample use the same tip for every well (Pipet slowly!)
98C 30 sec -> 20 cycles of (98C 10sec->64C 20sec->72C 20sec)->72C 5 min
Media:JD-cpg97k+30k-Breast_cancer-01230B09-Jan15_log.pdf Media:AL-Exome-CViB-Jan14-AB.pdf Media:AL-Exome-CViB-Jan14-CDE.pdf
Well / Set | C(t) | Difference | nM | ||
PhiX | A1 | 6.98 | 7.06 | ||
PhiX | B1 | 7.2 | |||
PhiX | C1 | 7.16 | |||
PhiX | D1 | 6.89 | |||
JD-cpg97k+30k-Breast_cancer-01230B09-Jan15 | E1 | 6.82 | 6.92 | 0.14 | 1.1 |
JD-cpg97k+30k-Breast_cancer-01230B09-Jan15 | F1 | 7.09 | |||
JD-cpg97k+30k-Breast_cancer-01230B09-Jan15 | G1 | 6.89 | |||
JD-cpg97k+30k-Breast_cancer-01230B09-Jan15 | H1 | 6.86 | |||