Calendar/2010-1-16: Difference between revisions
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==AL-Exome-CViB-Jan14-AB== | ==1/10 AL-Exome-CViB-Jan14-AB== | ||
{| {{table}} | {| {{table}} | ||
| ||Well / Set||C(t)||Ave.||Difference||nM | | ||Well / Set||C(t)||Ave.||Difference||nM | ||
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| PhiX||D1||8.56|||||| | | PhiX||D1||8.56|||||| | ||
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| AL-Exome-CViB-Jan14-AB||G2||8.13||7.99||0.71||1.64 | | 1/10 AL-Exome-CViB-Jan14-AB||G2||8.13||7.99||0.71||1.64 | ||
|- | |- | ||
| AL-Exome-CViB-Jan14-AB||H2||7.85 | | 1/10 AL-Exome-CViB-Jan14-AB||H2||7.85 | ||
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Revision as of 18:23, 21 January 2010
Quantify libraries with Phi-X ladder qPCR
AL-Exome-CViB-Jan14-AB w/ 1/10 dilution, AL-Exome-CViB-Jan14-CDE w/ 1/10 dilution, Ida's library
Setup | ||
1nm PhiX | AL-Exome-CViB-Jan14-AB | AL-Exome-CViB-Jan14-CDE |
1nm PhiX | AL-Exome-CViB-Jan14-AB | AL-Exome-CViB-Jan14-CDE |
1nm PhiX | AL-Exome-CViB-Jan14-AB | AL-Exome-CViB-Jan14-CDE |
1nm PhiX | AL-Exome-CViB-Jan14-AB | AL-Exome-CViB-Jan14-CDE |
Ida | 1/10 AL-Exome-CViB-Jan14-AB | 1/10 AL-Exome-CViB-Jan14-CDE |
Ida | 1/10 AL-Exome-CViB-Jan14-AB | 1/10 AL-Exome-CViB-Jan14-CDE |
Ida | 1/10 AL-Exome-CViB-Jan14-AB | 1/10 AL-Exome-CViB-Jan14-CDE |
Ida | 1/10 AL-Exome-CViB-Jan14-AB | 1/10 AL-Exome-CViB-Jan14-CDE |
2 Wells | X13 | |
DNA | 2 | 26 |
Syb_FP5 (100uM) | 0.2 | 2.6 |
Syb_RP7 (100uM) | 0.2 | 2.6 |
2X Phusion | 25 | , |
50X SYBR Green | 0.2 | 2.6 |
H2O | 24 | 312 |
Total | 345.8 | |
* Dilute sample, 1ul in 9ul ddh2o * mix 2ul 1/10 sample with 98ul mix in non-stick tube * aliquot 24ul to each well, for each sample use the same tip for every well (Pipet slowly!)
98C 30 sec -> 20 cycles of (98C 10sec->64C 20sec->72C 20sec)->72C 5 min
Media:JD-cpg97k+30k-Breast_cancer-01230B09-Jan15_log.pdf Media:AL-Exome-CViB-Jan14-AB.pdf Media:AL-Exome-CViB-Jan14-CDE.pdf
JD-cpg97k+30k-Breast_cancer-01230B09-Jan15
Well / Set | C(t) | Difference | nM | ||
PhiX | A1 | 6.98 | 7.06 | ||
PhiX | B1 | 7.2 | |||
PhiX | C1 | 7.16 | |||
PhiX | D1 | 6.89 | |||
JD-cpg97k+30k-Breast_cancer-01230B09-Jan15 | E1 | 6.82 | 6.92 | 0.14 | 1.1 |
JD-cpg97k+30k-Breast_cancer-01230B09-Jan15 | F1 | 7.09 | |||
JD-cpg97k+30k-Breast_cancer-01230B09-Jan15 | G1 | 6.89 | |||
JD-cpg97k+30k-Breast_cancer-01230B09-Jan15 | H1 | 6.86 | |||
AL-Exome-CViB-Jan14-AB
Well / Set | C(t) | Ave. | Difference | nM | |
PhiX | A1 | 7.99 | 8.11 | ||
PhiX | B1 | 8.33 | |||
PhiX | C1 | 8.18 | |||
PhiX | D1 | 7.95 | |||
AL-Exome-CViB-Jan14-AB | B2 | 4.48 | 4.51 | 3.61 | 12.17 |
AL-Exome-CViB-Jan14-AB | C2 | 4.57 | |||
AL-Exome-CViB-Jan14-AB | D2 | 4.47 | |||
1/10 AL-Exome-CViB-Jan14-AB
Well / Set | C(t) | Ave. | Difference | nM | |
PhiX | A1 | 8.55 | 8.7 | ||
PhiX | B1 | 8.96 | |||
PhiX | C1 | 8.74 | |||
PhiX | D1 | 8.56 | |||
1/10 AL-Exome-CViB-Jan14-AB | G2 | 8.13 | 7.99 | 0.71 | 1.64 |
1/10 AL-Exome-CViB-Jan14-AB | H2 | 7.85 | |||