AlanFung:Protocol/KAPA Library Quantification Kits-GA: Difference between revisions
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>Alan6017518 (Created page with '==Prepare qPCR Reagents== ===Ensure that the following reagents are completely thawed and thoroughly mixed by vortexing=== *2X KAPA SYBR FAST qPCR Master Mix *6 DNA Standards *1:…') |
>Alan6017518 No edit summary |
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==Step2== | ==Step2== | ||
==qPCR Setup== | |||
{| {{table}} | |||
| Setup||Volume(ul) | |||
|- | |||
| KAPA SYBR FAST qPCR Master Mix Containing Primer Premix||12 | |||
|- | |||
| PCR-grade water||4 | |||
|- | |||
| Diluted library DNA or DNA Standard (1-6)||4 | |||
|- | |||
| | |||
|} |
Revision as of 18:55, 20 January 2010
Prepare qPCR Reagents
Ensure that the following reagents are completely thawed and thoroughly mixed by vortexing
- 2X KAPA SYBR FAST qPCR Master Mix
- 6 DNA Standards
- 1:1000, 1:2000, 1:4000 & 1:8000 dilution of library DNA
Step1
- Perform an an 1:1000 dilution of the purified library in Library Dilution Buffer
- Optional 1:2000, 1:4000, 1:8000
Step2
qPCR Setup
Setup | Volume(ul) |
KAPA SYBR FAST qPCR Master Mix Containing Primer Premix | 12 |
PCR-grade water | 4 |
Diluted library DNA or DNA Standard (1-6) | 4 |