4th: Difference between revisions

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>Alan6017518
(/* Stock Phi-X (1nM),Stock Phi-X (0.1nM), Stock Phi-X (0.05nM), JD-cpg97k+30k-Breast_cancer-01230B09-Jan15,1/10 JD-cpg97k+30k-Breast_cancer-01230B09-Jan15, 1/10 AL-Exome-CViB-Jan14-AB, 1/100 AL-Exome-CViB-Jan14-AB, 1/10 AL-Exome-CViB-Jan14-CDE, 1/100)
>Alan6017518
(/* Stock Phi-X (1nM),Stock Phi-X (0.1nM), Stock Phi-X (0.05nM), JD-cpg97k+30k-Breast_cancer-01230B09-Jan15,1/10 JD-cpg97k+30k-Breast_cancer-01230B09-Jan15, 1/10 AL-Exome-CViB-Jan14-AB, 1/100 AL-Exome-CViB-Jan14-AB, 1/10 AL-Exome-CViB-Jan14-CDE, 1/100)
Line 15: Line 15:
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| Stock Phi-X (0.05nM)||1/100 AL-Exome-CViB-Jan14-AB||
| Stock Phi-X (0.05nM)||1/100 AL-Exome-CViB-Jan14-AB||
|-
|
|}
{| {{table}}
| ||For 2 wells||X10
|-
| DNA||2.000||
|-
| Syb_FP5 (100uM)||0.200||2.000
|-
| Syb_RP7 (100uM)||0.200||2.000
|-
| 2X Phusion||25.000||250.000
|-
| 50X SYBR Green I||0.200||2.000
|-
| H20||24.000||240.000
|-
|-
|  
|  
|}
|}

Revision as of 18:58, 21 January 2010

Quantify libraries with Phi-X ladder qPCR

Stock Phi-X (1nM),Stock Phi-X (0.1nM), Stock Phi-X (0.05nM), JD-cpg97k+30k-Breast_cancer-01230B09-Jan15,1/10 JD-cpg97k+30k-Breast_cancer-01230B09-Jan15, 1/10 AL-Exome-CViB-Jan14-AB, 1/100 AL-Exome-CViB-Jan14-AB, 1/10 AL-Exome-CViB-Jan14-CDE, 1/100 AL-Exome-CViB-Jan14-CDE

Setup
Stock Phi-X (1nM) 1/10 JD-cpg97k+30k-Breast_cancer-01230B09-Jan15 1/100 AL-Exome-CViB-Jan14-CDE
Stock Phi-X (1nM) 1/10JD-cpg97k+30k-Breast_cancer-01230B09-Jan15 1/100 AL-Exome-CViB-Jan14-CDE
Stock Phi-X (0.1nM) 1/10 AL-Exome-CViB-Jan14-AB
Stock Phi-X (0.1nM) 1/10 AL-Exome-CViB-Jan14-AB
Stock Phi-X (0.05nM) 1/100 AL-Exome-CViB-Jan14-AB
Stock Phi-X (0.05nM) 1/100 AL-Exome-CViB-Jan14-AB
For 2 wells X10
DNA 2.000
Syb_FP5 (100uM) 0.200 2.000
Syb_RP7 (100uM) 0.200 2.000
2X Phusion 25.000 250.000
50X SYBR Green I 0.200 2.000
H20 24.000 240.000