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>Alan6017518
(/* Stock Phi-X (1nM),Stock Phi-X (0.1nM), Stock Phi-X (0.05nM), JD-cpg97k+30k-Breast_cancer-01230B09-Jan15,1/10 JD-cpg97k+30k-Breast_cancer-01230B09-Jan15, 1/10 AL-Exome-CViB-Jan14-AB, 1/100 AL-Exome-CViB-Jan14-AB, 1/10 AL-Exome-CViB-Jan14-CDE, 1/100)
>Alan6017518
(Blanked the page)
 
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==Quantify libraries with Phi-X ladder qPCR==
==Stock Phi-X (1nM),Stock Phi-X (0.1nM), Stock Phi-X (0.05nM), JD-cpg97k+30k-Breast_cancer-01230B09-Jan15,1/10 JD-cpg97k+30k-Breast_cancer-01230B09-Jan15, 1/10 AL-Exome-CViB-Jan14-AB, 1/100 AL-Exome-CViB-Jan14-AB, 1/10 AL-Exome-CViB-Jan14-CDE, 1/100 AL-Exome-CViB-Jan14-CDE==
{| {{table}}
| Setup||||
|-
| Stock Phi-X (1nM)||1/10 JD-cpg97k+30k-Breast_cancer-01230B09-Jan15||1/100 AL-Exome-CViB-Jan14-CDE
|-
| Stock Phi-X (1nM)||1/10JD-cpg97k+30k-Breast_cancer-01230B09-Jan15||1/100 AL-Exome-CViB-Jan14-CDE
|-
| Stock Phi-X (0.1nM)||1/10 AL-Exome-CViB-Jan14-AB||
|-
| Stock Phi-X (0.1nM)||1/10 AL-Exome-CViB-Jan14-AB||
|-
| Stock Phi-X (0.05nM)||1/100 AL-Exome-CViB-Jan14-AB||
|-
| Stock Phi-X (0.05nM)||1/100 AL-Exome-CViB-Jan14-AB||
|-
|
|}
{| {{table}}
| ||For 2 wells||X10
|-
| DNA||2.000||
|-
| Syb_FP5 (100uM)||0.200||2.000
|-
| Syb_RP7 (100uM)||0.200||2.000
|-
| 2X Phusion||25.000||250.000
|-
| 50X SYBR Green I||0.200||2.000
|-
| H20||24.000||240.000
|-
|
|}
    * Dilute sample, 1ul in 9ul ddh2o
    * mix 2ul 1/10 sample with 98ul mix in non-stick tube
    * aliquot 24ul to each well, for each sample use the same tip for every well (Pipet slowly!)


98C 30 sec -> 20 cycles of (98C 10sec->64C 20sec->72C 20sec)->72C 5 min

Latest revision as of 21:42, 21 January 2010