Alice:LabNotes/2010-1-21: Difference between revisions

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>Zsakura2
(Created page with '==MmeI digestion on CViB sequencing libraries== *Previous library construction protocol can be found under labnotes 1-9-10 *size selection protocol can found under labnotes 1-15-…')
 
>Zsakura2
Line 5: Line 5:
  setup:
  setup:
  DNA(15nM)                  10ul
  DNA(15nM)                  10ul
  10x buffer 4                 2ul
  10x buffer 4               2ul
  1mM SAM                     2ul
  1mM SAM                     2ul
  MmeI(2U/ul)                2ul
  MmeI(2U/ul)                2ul
  37C 1hr, 80C 20min
  37C 1hr, 80C 20min

Revision as of 21:54, 21 January 2010

MmeI digestion on CViB sequencing libraries

  • Previous library construction protocol can be found under labnotes 1-9-10
  • size selection protocol can found under labnotes 1-15-10
setup:
DNA(15nM)                   10ul
10x buffer 4                2ul
1mM SAM                     2ul
MmeI(2U/ul)                 2ul
37C 1hr, 80C 20min