Alice:LabNotes/2010-1-21: Difference between revisions
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>Zsakura2 (Created page with '==MmeI digestion on CViB sequencing libraries== *Previous library construction protocol can be found under labnotes 1-9-10 *size selection protocol can found under labnotes 1-15-…') |
>Zsakura2 |
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Line 5: | Line 5: | ||
setup: | setup: | ||
DNA(15nM) 10ul | DNA(15nM) 10ul | ||
10x buffer 4 | 10x buffer 4 2ul | ||
1mM SAM | 1mM SAM 2ul | ||
MmeI(2U/ul) 2ul | MmeI(2U/ul) 2ul | ||
37C 1hr, 80C 20min | 37C 1hr, 80C 20min |
Revision as of 21:54, 21 January 2010
MmeI digestion on CViB sequencing libraries
- Previous library construction protocol can be found under labnotes 1-9-10
- size selection protocol can found under labnotes 1-15-10
setup: DNA(15nM) 10ul 10x buffer 4 2ul 1mM SAM 2ul MmeI(2U/ul) 2ul 37C 1hr, 80C 20min