Sam:LabNotes/Human-Chip-bisulfite-Seq/2010-2-15 exp2: Difference between revisions

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*Test low-input DNA libaray conscturction using internal marker at 200ng, 50ng, 10ng at 0ng, with or without De-P-carrier.
*Test low-input DNA libaray conscturction using internal marker at 200ng, 50ng, 10ng at 0ng, with or without De-P-carrier.
*Goals:
*Goals:
**Test the newly synthesized adaptors and primers.
**Test the newly synthesized adaptors and primers by myself.
**Test Celso's protocol (with new End-repaing recipe).
**Test Celso's protocol (with new End-repaing recipe).
**Get the refence position of post-ligation reactions from internal marker template.
**Get the refence position of post-ligation reactions from internal marker template.

Revision as of 05:37, 16 February 2010

Low-Input DNA libarary construction - Internal marker templates

Background

  • Test low-input DNA libaray conscturction using internal marker at 200ng, 50ng, 10ng at 0ng, with or without De-P-carrier.
  • Goals:
    • Test the newly synthesized adaptors and primers by myself.
    • Test Celso's protocol (with new End-repaing recipe).
    • Get the refence position of post-ligation reactions from internal marker template.
    • Compare the effect the adding De-P-carrier or not on library construction.

Pocedures