Sam:LabNotes/Human-Chip-bisulfite-Seq/2010-2-15 exp2: Difference between revisions
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*Test low-input DNA libaray conscturction using internal marker at 200ng, 50ng, 10ng at 0ng, with or without De-P-carrier. | *Test low-input DNA libaray conscturction using internal marker at 200ng, 50ng, 10ng at 0ng, with or without De-P-carrier. | ||
*Goals: | *Goals: | ||
**Test the newly synthesized adaptors and primers. | **Test the newly synthesized adaptors and primers by myself. | ||
**Test Celso's protocol (with new End-repaing recipe). | **Test Celso's protocol (with new End-repaing recipe). | ||
**Get the refence position of post-ligation reactions from internal marker template. | **Get the refence position of post-ligation reactions from internal marker template. |
Revision as of 05:37, 16 February 2010
Low-Input DNA libarary construction - Internal marker templates
Background
- Test low-input DNA libaray conscturction using internal marker at 200ng, 50ng, 10ng at 0ng, with or without De-P-carrier.
- Goals:
- Test the newly synthesized adaptors and primers by myself.
- Test Celso's protocol (with new End-repaing recipe).
- Get the refence position of post-ligation reactions from internal marker template.
- Compare the effect the adding De-P-carrier or not on library construction.