Sam:LabNotes/Human-Chip-bisulfite-Seq/2010-2-15 exp2: Difference between revisions
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**Test Celso's protocol (with new End-repaing recipe). | **Test Celso's protocol (with new End-repaing recipe). | ||
**Get the refence position of post-ligation reactions from internal marker template. | **Get the refence position of post-ligation reactions from internal marker template. | ||
**Compare the effect | **Compare the effect of adding De-P-carrier or not on library construction. | ||
==Pocedures== | ==Pocedures== |
Revision as of 05:37, 16 February 2010
Low-Input DNA libarary construction - Internal marker templates
Background
- Test low-input DNA libaray conscturction using internal marker at 200ng, 50ng, 10ng at 0ng, with or without De-P-carrier.
- Goals:
- Test the newly synthesized adaptors and primers by myself.
- Test Celso's protocol (with new End-repaing recipe).
- Get the refence position of post-ligation reactions from internal marker template.
- Compare the effect of adding De-P-carrier or not on library construction.