AlanFung:LabNotes/CTCF/2010-3-24: Difference between revisions

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>Alan6017518
>Alan6017518
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  Gel Quant: 18.95ng/ul
  Gel Quant: 18.95ng/ul
[[File:ZhangLab_2 2010-03-24 11hr 35min.jpg]]
[[File:ZhangLab_2 2010-03-24 11hr 35min.jpg]]
* I think both the nanaodrop and gel quantification are biased, for the nanaodrop it gives out an extremely high amount of concentration, this usually happens because nanaodrop cannot differentiate between any contamination. As for the gel image the band at 100bp is much stronger than the ladder band which hits that the concentration is higher than 18.95ng/ul. In this case I would go with the qubit quantification.

Revision as of 20:56, 24 March 2010

Quantification of Jurkat PCR Product

Nanodrop: 250.7ng/ul
Qubit: 48.4ng/ul
Gel Quant: 18.95ng/ul

File:ZhangLab 2 2010-03-24 11hr 35min.jpg

  • I think both the nanaodrop and gel quantification are biased, for the nanaodrop it gives out an extremely high amount of concentration, this usually happens because nanaodrop cannot differentiate between any contamination. As for the gel image the band at 100bp is much stronger than the ladder band which hits that the concentration is higher than 18.95ng/ul. In this case I would go with the qubit quantification.