Dinh/NOTES/2010-7-3: Difference between revisions
Jump to navigation
Jump to search
>Dinh mNo edit summary |
>Dinh mNo edit summary |
||
(5 intermediate revisions by the same user not shown) | |||
Line 4: | Line 4: | ||
LinkerR-LMNA-F: GTT GGA GGC TCA TCG TTC CTA TTT TGG GCC TGA GTG GTC AGT C | LinkerR-LMNA-F: GTT GGA GGC TCA TCG TTC CTA TTT TGG GCC TGA GTG GTC AGT C | ||
LinkerL-LMNA-R: GAC CCG CCT GCA GGA TTT GGC AGG CAG ATG TTA TCG AGG TCC GAC | LinkerL-LMNA-R: GAC CCG CCT GCA GGA TTT GGC AGG CAG ATG TTA TCG AGG TCC GAC | ||
Product length: 373+ | Product length: 373+23bp+25bp= 421bp | ||
Map to position: chr1:156,108,195-156,108,590 | |||
===PCR=== | ===PCR=== | ||
Line 15: | Line 15: | ||
----------------------- | ----------------------- | ||
50ul | 50ul | ||
Templates used: | Templates used: HGPS-ips | ||
Program: | Program: | ||
Line 29: | Line 29: | ||
15C forever | 15C forever | ||
==Results== | |||
===PAGE=== | |||
[[File:Attaching linker to LMNA amplicons.jpg]] | |||
== | ===LinkerR-LMNA-R sequence was wrong=== |
Latest revision as of 21:19, 9 July 2010
Sanger sequencing of PCR product with probe linker[edit]
Primers[edit]
LinkerR-LMNA-F: GTT GGA GGC TCA TCG TTC CTA TTT TGG GCC TGA GTG GTC AGT C LinkerL-LMNA-R: GAC CCG CCT GCA GGA TTT GGC AGG CAG ATG TTA TCG AGG TCC GAC Product length: 373+23bp+25bp= 421bp Map to position: chr1:156,108,195-156,108,590
PCR[edit]
Template (1-2ng/ul): 3ul F primer (10uM) : 1ul R primer (10uM) : 1ul H20 : 20ul Taq 2x MM : 25ul ----------------------- 50ul Templates used: HGPS-ips
Program: 94C 2min 94C 30s 62C 40s 72C 40s Go to 2 for 7x 94C 30s 72C 40s Go to 7 for 23x 72C 3min 15C forever
Results[edit]
PAGE[edit]
File:Attaching linker to LMNA amplicons.jpg