Kun:LabNotes/GenomeSeq/2010-8-20: Difference between revisions

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(Created page with '==Ep gDNA== *Received three DNA samples in dry pallets from XMU. *Resuspend the DNA in each tube with 100ul EB. *Measure concentration with Nanodrop: **EP-G1: 63ng/ul x 100ul **E…')
 
 
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==Ep gDNA==
==Ep gDNA==
*Received three DNA samples in dry pallets from XMU.
*Received three DNA Epigonichthys samples in dry pallets from XMU.
*Resuspend the DNA in each tube with 100ul EB.
*Resuspend the DNA in each tube with 100ul EB.
*Measure concentration with Nanodrop:
*Measure concentration with Nanodrop:
Line 6: Line 6:
**EP-G2: 36ng/ul x 100ul
**EP-G2: 36ng/ul x 100ul
**EP-G3: 134ng/ul x 100ul
**EP-G3: 134ng/ul x 100ul
==Prepare DNA for covaris shearing==
                              300bp          800bp
    B.Jap-1 (110ng/ul)          20ul          40ul   
    B.Jap-2 (105ng/ul)          20ul          40ul
    B.Jap-3 (457ng/ul)          20ul          40ul
    Ep.G-1  (65ng/ul)          40ul          60ul
    Ep.G-2  (36ng/ul)          100ul
    Ep.G-3  (134ng/ul)          30ul          70ul

Latest revision as of 18:17, 24 August 2010

Ep gDNA[edit]

  • Received three DNA Epigonichthys samples in dry pallets from XMU.
  • Resuspend the DNA in each tube with 100ul EB.
  • Measure concentration with Nanodrop:
    • EP-G1: 63ng/ul x 100ul
    • EP-G2: 36ng/ul x 100ul
    • EP-G3: 134ng/ul x 100ul

Prepare DNA for covaris shearing[edit]

                              300bp          800bp
   B.Jap-1 (110ng/ul)          20ul          40ul     
   B.Jap-2 (105ng/ul)          20ul          40ul
   B.Jap-3 (457ng/ul)          20ul          40ul
   Ep.G-1  (65ng/ul)           40ul          60ul
   Ep.G-2  (36ng/ul)          100ul
   Ep.G-3  (134ng/ul)          30ul          70ul