AlanFung:LabNotes/ASE/2010/2010-9-13: Difference between revisions
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*3)Combine files | *3)Combine files | ||
*Use Excel to create script for merging ID filles from serval lanes of Data to one file | |||
*4)Run aseBowtieSam.pl | *4)Run aseBowtieSam.pl | ||
*5)Run baseCalls2Ase.pl on local machine | *5)Run baseCalls2Ase.pl on local machine |
Revision as of 22:03, 13 September 2010
Analysis of the Hues6/BJ hybrid data
Summary of experiments
Index Table
gDNA | cDNA | |||||
HL068_2 | HL071 | HL068_2 | HL071 | |||
5+6 | 5 + 6 | 7+8 | 7+8 | |||
IND1 | N/A | H1 gDNA | IND1 | 1000s | 1000s | |
IND2 | 1000ips | 1000ips | IND2 | 1000ips | 1000ips | |
IND3 | 1034ips | 1034ips | IND3 | 1034ips | 1034ips | |
IND4 | A2 | A2 | IND4 | A2 | A2 | |
IND5 | B4 | B4 | IND5 | B4 | B4 | |
IND6 | 1016ips | 1016ips | IND6 | 1016s | 1016s | |
IND7 | N/A | N/A | IND7 | 1016ips | 1016ips | |
IND8 | B5 | B5 | IND8 | B5ips | B5ips | |
IND9 | Hues6 | Hues6 | IND9 | Hues6 | Hues6 | |
IND10 | N/A | N/A | IND10 | BJ | BJ | |
IND11 | BJ | BJ | IND11 | N/A | 1034s | |
IND12 | N/A | H1 cDNA | IND12 | N/A | N/A | |
Wiki | KZ 7_22 | HL 8_26 | HL 7_26 | HL 8_26 |
Workflow
- 1) Convert using BCL converter
- 2)Demultiplex
- Script for demultiplex
- 3)Combine files
- Use Excel to create script for merging ID filles from serval lanes of Data to one file
- 4)Run aseBowtieSam.pl
- 5)Run baseCalls2Ase.pl on local machine