AlanFung:LabNotes/ASE/2010/2010-9-13: Difference between revisions
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3)Combine files | 3)Combine files | ||
Use Excel to create script for merging ID filles from serval lanes of Data to one file | |||
Revision as of 22:03, 13 September 2010
Analysis of the Hues6/BJ hybrid data
Summary of experiments
Index Table
gDNA | cDNA | |||||
HL068_2 | HL071 | HL068_2 | HL071 | |||
5+6 | 5 + 6 | 7+8 | 7+8 | |||
IND1 | N/A | H1 gDNA | IND1 | 1000s | 1000s | |
IND2 | 1000ips | 1000ips | IND2 | 1000ips | 1000ips | |
IND3 | 1034ips | 1034ips | IND3 | 1034ips | 1034ips | |
IND4 | A2 | A2 | IND4 | A2 | A2 | |
IND5 | B4 | B4 | IND5 | B4 | B4 | |
IND6 | 1016ips | 1016ips | IND6 | 1016s | 1016s | |
IND7 | N/A | N/A | IND7 | 1016ips | 1016ips | |
IND8 | B5 | B5 | IND8 | B5ips | B5ips | |
IND9 | Hues6 | Hues6 | IND9 | Hues6 | Hues6 | |
IND10 | N/A | N/A | IND10 | BJ | BJ | |
IND11 | BJ | BJ | IND11 | N/A | 1034s | |
IND12 | N/A | H1 cDNA | IND12 | N/A | N/A | |
Wiki | KZ 7_22 | HL 8_26 | HL 7_26 | HL 8_26 |
Workflow
1) Convert using BCL converter
2)Demultiplex Script for demultiplex
3)Combine files Use Excel to create script for merging ID filles from serval lanes of Data to one file
4)Run aseBowtieSam.pl
5)Run baseCalls2Ase.pl on local machine