Sam:LabNotes/Microbiome-new/2010-9-24: Difference between revisions

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==Proceduress==
==Proceduress==
*Sample: E.coli gDNA MDA amplicons made on 09-10-'10 (666ng/uL)
**http://genome-tech.ucsd.edu/LabNotes/index.php/Sam:LabNotes/Microbiome-new/2010-9-10
*S1 nuclease digestion (debranching)
**http://genome-tech.ucsd.edu/LabNotes/index.php/Sam:LabNotes/Microbiome-new/2010-9-7

Revision as of 15:39, 25 September 2010

Sanger sequencing test on E.coli gDNA MDA amplicons

Background

  • To optimize the Sanger sequencing protocl for MDA amplicon validation
  • This protocol will also include the S1 nucelase debranching, DNA Pol.I reparing procedures, and compare the difference between with and without these treatments.

Proceduress