Sam:LabNotes/Microbiome-new/2010-9-24: Difference between revisions

From ZhangLabWiki
Jump to navigation Jump to search
>Sam Chiang
>Sam Chiang
No edit summary
Line 5: Line 5:
*This protocol will also include the S1 nucelase debranching, DNA Pol.I reparing procedures, and compare the difference between with and without these treatments.
*This protocol will also include the S1 nucelase debranching, DNA Pol.I reparing procedures, and compare the difference between with and without these treatments.


==Proceduress==
==Procedures==
*Sample: E.coli gDNA MDA amplicons made on 09-10-'10 (666ng/uL)
*Sample: E.coli gDNA MDA amplicons made on 09-10-'10 (666ng/uL)
**http://genome-tech.ucsd.edu/LabNotes/index.php/Sam:LabNotes/Microbiome-new/2010-9-10
**http://genome-tech.ucsd.edu/LabNotes/index.php/Sam:LabNotes/Microbiome-new/2010-9-10

Revision as of 15:40, 25 September 2010

Sanger sequencing test on E.coli gDNA MDA amplicons

Background

  • To optimize the Sanger sequencing protocl for MDA amplicon validation
  • This protocol will also include the S1 nucelase debranching, DNA Pol.I reparing procedures, and compare the difference between with and without these treatments.

Procedures