Sam:LabNotes/Microbiome-new/2010-9-24: Difference between revisions
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*This protocol will also include the S1 nucelase debranching, DNA Pol.I reparing procedures, and compare the difference between with and without these treatments. | *This protocol will also include the S1 nucelase debranching, DNA Pol.I reparing procedures, and compare the difference between with and without these treatments. | ||
== | ==Procedures== | ||
*Sample: E.coli gDNA MDA amplicons made on 09-10-'10 (666ng/uL) | *Sample: E.coli gDNA MDA amplicons made on 09-10-'10 (666ng/uL) | ||
**http://genome-tech.ucsd.edu/LabNotes/index.php/Sam:LabNotes/Microbiome-new/2010-9-10 | **http://genome-tech.ucsd.edu/LabNotes/index.php/Sam:LabNotes/Microbiome-new/2010-9-10 |
Revision as of 15:40, 25 September 2010
Sanger sequencing test on E.coli gDNA MDA amplicons
Background
- To optimize the Sanger sequencing protocl for MDA amplicon validation
- This protocol will also include the S1 nucelase debranching, DNA Pol.I reparing procedures, and compare the difference between with and without these treatments.
Procedures
- Sample: E.coli gDNA MDA amplicons made on 09-10-'10 (666ng/uL)
- S1 nuclease digestion (debranching)