Noi:LabNotes/2010-10-21: Difference between revisions

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(Created page with '==Probe prep for the DMR220k_S4 and S5== (followed Dr. Zhang's conditions) ===Production PCR=== 10nM 1st …')
 
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==Probe prep for the DMR220k_S4 and S5==
(followed Dr. Zhang's conditions)


===Production PCR===
                                                                     
10nM 1st round amplicon      0.2ul               
    2x Kapa SYBG qPCR Master Mix  50ul       
    100uM pAP1V61U              0.4ul                 
    100uM AP2V6                  0.4ul                     
    H2O                          49ul                         
    ===================================
                                100ul x 96
    95C 30sec -> (95C 3sec -> 55C 30sec-> 60C 20sec) x 16 -> 60C 2min -> 15C hold.
    Ethanol precipitation; re-purified with twelve Qiaquick columns, each eluted with 40ul EB.
   
                                                                        DMR220k_S4                                            DMR220k_S5
    DNA concentration (ng/ul)                                                                     
    O.D.260/280
    Yield: concxtotal vol.

Latest revision as of 09:22, 28 October 2010