AlanFung:LabNotes/Reprogramming/2010-11-10: Difference between revisions

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(Created page with '==one step qRT-PCR== *The purpose of this experiment is to perform quantitative RT-PCR for the detection of the efficiency of SPEN gene knockdown.')
 
>Alan6017518
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==one step qRT-PCR==
==one step qRT-PCR==
*The purpose of this experiment is to perform quantitative RT-PCR for the detection of the efficiency of SPEN gene knockdown.
*The purpose of this experiment is to perform quantitative RT-PCR for the detection of the efficiency of SPEN gene knockdown.
Running at Program “PCR62” (Tm=62, 30cycle)
50C 3min -> hold -> 95C 5min -> hold (95C 15sec -> 60C 30sec) x 40 cycle -> 40C 1min

Revision as of 23:15, 9 November 2010

one step qRT-PCR

  • The purpose of this experiment is to perform quantitative RT-PCR for the detection of the efficiency of SPEN gene knockdown.

Running at Program “PCR62” (Tm=62, 30cycle) 50C 3min -> hold -> 95C 5min -> hold (95C 15sec -> 60C 30sec) x 40 cycle -> 40C 1min