AlanFung:LabNotes/Reprogramming/2010-11-10: Difference between revisions
Jump to navigation
Jump to search
>Alan6017518 (Created page with '==one step qRT-PCR== *The purpose of this experiment is to perform quantitative RT-PCR for the detection of the efficiency of SPEN gene knockdown.') |
>Alan6017518 |
||
Line 1: | Line 1: | ||
==one step qRT-PCR== | ==one step qRT-PCR== | ||
*The purpose of this experiment is to perform quantitative RT-PCR for the detection of the efficiency of SPEN gene knockdown. | *The purpose of this experiment is to perform quantitative RT-PCR for the detection of the efficiency of SPEN gene knockdown. | ||
Running at Program “PCR62” (Tm=62, 30cycle) | |||
50C 3min -> hold -> 95C 5min -> hold (95C 15sec -> 60C 30sec) x 40 cycle -> 40C 1min |
Revision as of 23:15, 9 November 2010
one step qRT-PCR
- The purpose of this experiment is to perform quantitative RT-PCR for the detection of the efficiency of SPEN gene knockdown.
Running at Program “PCR62” (Tm=62, 30cycle) 50C 3min -> hold -> 95C 5min -> hold (95C 15sec -> 60C 30sec) x 40 cycle -> 40C 1min