Sam:LabNotes/Microbiome-new/2010-11-15: Difference between revisions

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>Sam Chiang
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**http://genome-tech.ucsd.edu/LabNotes/index.php/Sam:LabNotes/Microbiome-new/2010-11-14
**http://genome-tech.ucsd.edu/LabNotes/index.php/Sam:LabNotes/Microbiome-new/2010-11-14
*The pool library will be size-selected again in order to get a narrower band for sequencing.
*The pool library will be size-selected again in order to get a narrower band for sequencing.
==Procedures==
*The pooled library is ~40uL. The pooled library was seperated into two tube (20uL/tube) for size-selection using 5-well TBE-PAGE.
**Sample well: 10uL H2O + 10uL 6X loading dye + 20uL DNA sample
**Ladder well: 30uL H2O + 10uL 6X loading dye + 0.5uL Low mass ladder/1kb plus ladder
*Run at 200V for 30min. Long staining (15min with foil wrap protection).
[[File:sam111510.jpg]]

Revision as of 07:20, 16 November 2010

2nd size selection of pooled Illumina library(short and long amplified E.coli SAGs -3rd test 11-07-'10)

Background

Procedures

  • The pooled library is ~40uL. The pooled library was seperated into two tube (20uL/tube) for size-selection using 5-well TBE-PAGE.
    • Sample well: 10uL H2O + 10uL 6X loading dye + 20uL DNA sample
    • Ladder well: 30uL H2O + 10uL 6X loading dye + 0.5uL Low mass ladder/1kb plus ladder
  • Run at 200V for 30min. Long staining (15min with foil wrap protection).
File:Sam111510.jpg