Sam:LabNotes/Microbiome-new/2010-11-15: Difference between revisions
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**http://genome-tech.ucsd.edu/LabNotes/index.php/Sam:LabNotes/Microbiome-new/2010-11-14 | **http://genome-tech.ucsd.edu/LabNotes/index.php/Sam:LabNotes/Microbiome-new/2010-11-14 | ||
*The pool library will be size-selected again in order to get a narrower band for sequencing. | *The pool library will be size-selected again in order to get a narrower band for sequencing. | ||
==Procedures== | |||
*The pooled library is ~40uL. The pooled library was seperated into two tube (20uL/tube) for size-selection using 5-well TBE-PAGE. | |||
**Sample well: 10uL H2O + 10uL 6X loading dye + 20uL DNA sample | |||
**Ladder well: 30uL H2O + 10uL 6X loading dye + 0.5uL Low mass ladder/1kb plus ladder | |||
*Run at 200V for 30min. Long staining (15min with foil wrap protection). | |||
[[File:sam111510.jpg]] |
Revision as of 07:20, 16 November 2010
2nd size selection of pooled Illumina library(short and long amplified E.coli SAGs -3rd test 11-07-'10)
Background
- Eight indexed indivudual libraries were pooled based on equal amount (20ng)
- The pool library will be size-selected again in order to get a narrower band for sequencing.
Procedures
- The pooled library is ~40uL. The pooled library was seperated into two tube (20uL/tube) for size-selection using 5-well TBE-PAGE.
- Sample well: 10uL H2O + 10uL 6X loading dye + 20uL DNA sample
- Ladder well: 30uL H2O + 10uL 6X loading dye + 0.5uL Low mass ladder/1kb plus ladder
- Run at 200V for 30min. Long staining (15min with foil wrap protection).
File:Sam111510.jpg