Sam:LabNotes/Microbiome-new/2010-11-17: Difference between revisions

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==QUBIT Sample quantification==
==QUBIT Sample quantification==
  [[File:sam111710-qubit.jpg|400px]]
  [[File:sam111710-qubit.jpg|900px]]





Revision as of 07:26, 19 November 2010

MDA samples preparation for Covaris shearing-(single E.coli amplicons)

Background

QUBIT Sample quantification

File:Sam111710-qubit.jpg


Procedures

  • Label the Covaris microtubes. Open the caps and leave tubes on 96-well rack.
  • UV-treat the tubes and pipettes for 15min.
    • Thaw the tRNA carrier (stored in -80C). Use the "2"-tRNA(96ng/uL)
 Sample no.   Sample name          Conc.(ng/uL)  Sample(uL)   330ng-tRNA("2")   H2O    Total DNA(ng)
 ---------------------------------------------------------------------------------------------------
 HC          sam080610_2-1_2h     0.685         100          3.43              0      68.5ng
 2-S          sam080610_2-2_2h     0.703         100          3.43              0      70.3ng
 3-S          sam081210_1-5_2h     0.704         100          3.43              0      70.4ng
 4-S          sam081210_2-5_2h     0.693         100          3.43              0      69.3ng
 
 1-L          sam080610_2-1_10h  220.0           2.27           -              97.7   500.0ng
 2-L          sam080610_2-2_10h  322.0           1.55           -              98.5   500.0ng
 3-L          sam081210_1-5_10h  540.0           0.92           -              99.0   500.0ng
 4-L          sam081210_2-5_10h  551.0           0.90           -              99.0   500.0ng
  • 100uL sample suspension were carefully transferred into microtubes.
  • Samples tubes were tapped on the rack and packed in a paper box with three disposable cooler.
  • The package was shipped to the Sara through FedEx(track# 4490-5094-2339 on 10-19-'10).